用于炎症生物标志物组合检测的全电子尿液试纸条探头

Fully electronic urine dipstick probe for combinatorial detection of inflammatory biomarkers.

作者信息

Kamakoti Vikramshankar, Kinnamon David, Choi Kang Hyeok, Jagannath Badrinath, Prasad Shalini

机构信息

Department of Bioengineering, University of Texas at Dallas, 800 W Campbell Rd, Richardson, TX 75080, USA.

出版信息

Future Sci OA. 2018 Mar 27;4(5):FSO301. doi: 10.4155/fsoa-2017-0142. eCollection 2018 Jun.

AIM

An electrochemical urine dipstick probe biosensor has been demonstrated using molybdenum electrodes on nanoporous polyamide substrate for the quantitative detection of two inflammatory protein biomarkers, CRP and IL-6.

MATERIALS & METHODS: The electrode interface was characterized using ζ-potential and Fourier transform infrared spectroscopy. Detection of biomarkers was demonstrated by measuring impedance changes associated with the dose concentrations of the two biomarkers. A proof of feasibility of point-of-care implementation of the biosensor was demonstrated using a portable electronics platform.

RESULTS & CONCLUSION: Limit of detection of 1 pg/ml was achieved for CRP and IL-6 in human urine and synthetic urine buffers. The developed portable hardware demonstrated close correlation with benchtop equipment results.