Spooner Bioanalytical Solutions Ltd., Hertford, UK & School of Life and Medical Sciences, University of Hertfordshire, UK.
Pharmaron UK Ltd., Rushden, Northamptonshire, UK.
J Pharm Biomed Anal. 2018 Feb 5;149:419-424. doi: 10.1016/j.jpba.2017.11.036. Epub 2017 Nov 14.
When using dried blood spot (DBS) sampling for the quantitative bioanalysis of circulating concentrations of drugs, metabolites and endogenous analytes, it is important that a fixed volume of blood is deposited to overcome the issues associated with blood hematocrit (HCT) and homogeneity. The volumetric performance of the KTH DBS collection device was tested with radiolabelled [C]-diclofenac. It was demonstrated that the device deposits a fixed volume of blood (13.5μL) regardless of the HCT (25-65%), or lipid content of the blood sample. Further, it was found that the precision and accuracy of the derived dried blood samples were at least as good as those of a positive displacement pipette. The device was found to be easy to use and gave acceptable dried samples for 92.9% of the tests performed (n=42).
当使用干血斑（DBS）采样进行药物、代谢物和内源性分析物的循环浓度定量生物分析时，重要的是要固定体积的血液沉积，以克服与血液血细胞比容（HCT）和均一性相关的问题。使用放射性标记的 [C]-双氯芬酸对 KTH DBS 收集装置的体积性能进行了测试。结果表明，该装置无论血液的 HCT（25-65%）或脂质含量如何，均能沉积固定体积的血液（13.5μL）。此外，还发现衍生的干血斑的精密度和准确度至少与正位移移液器一样好。该装置易于使用，在进行的 42 次测试中有 92.9%（n=42）获得了可接受的干血斑样本。
