Colagiorgi Angelo, Turroni Francesca, Mancabelli Leonardo, Serafini Fausta, Secchi Andrea, van Sinderen Douwe, Ventura Marco
Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, 43124 Parma, Italy.
Department of Chemistry, University of Parma, 43124 Parma, Italy.
FEMS Microbiol Lett. 2015 Sep;362(17):fnv141. doi: 10.1093/femsle/fnv141. Epub 2015 Aug 21.
Bifidobacteria are colonizers of the human gut, where they are interacting with their host as well as with other members of the intestinal microbiota. Teichoic acids (TAs) have previously been shown to play an important role in modulating microbe-host interactions in the human gut. However, so far, there is a paucity of information regarding the presence of TAs in the cell envelope of bifidobacteria. In silico analyses targeting the chromosomes of all 48 (sub)species that currently represent the genus Bifidobacterium revealed the presence of genes responsible for TA biosynthesis, suggesting that bifidobacteria contain both wall TAs and lipoteichoic acids. Transcriptome analyses of the infant gut commensal Bifidobacterium bifidum PRL2010 highlighted that the transcription of the presumptive TA biosynthetic loci is modulated in response to environmental conditions reflecting those of the human gut. Furthermore, chemical characterization of TAs produced by PRL2010 indicates the presence of lipoteichoic acids.
双歧杆菌是人类肠道的定植菌，在肠道中它们与宿主以及肠道微生物群的其他成员相互作用。磷壁酸（TAs）此前已被证明在调节人类肠道中的微生物-宿主相互作用方面发挥重要作用。然而，到目前为止，关于双歧杆菌细胞壁中磷壁酸的存在情况，相关信息匮乏。针对目前代表双歧杆菌属的所有48个（亚）种的染色体进行的计算机分析显示，存在负责磷壁酸生物合成的基因，这表明双歧杆菌同时含有壁磷壁酸和脂磷壁酸。对婴儿肠道共生菌两歧双歧杆菌PRL2010的转录组分析突出表明，假定的磷壁酸生物合成基因座的转录会根据反映人类肠道环境条件的变化而受到调节。此外，对PRL2010产生的磷壁酸进行化学表征表明存在脂磷壁酸。
