Laboratory of Probiogenomics, Department of Genetics, Biology of Microorganisms, Anthropology and Evolution, University of Parma, 43100 Parma, Italy.
Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19514-9. doi: 10.1073/pnas.1011100107. Epub 2010 Oct 25.
The human intestine is densely populated by a microbial consortium whose metabolic activities are influenced by, among others, bifidobacteria. However, the genetic basis of adaptation of bifidobacteria to the human gut is poorly understood. Analysis of the 2,214,650-bp genome of Bifidobacterium bifidum PRL2010, a strain isolated from infant stool, revealed a nutrient-acquisition strategy that targets host-derived glycans, such as those present in mucin. Proteome and transcriptome profiling revealed a set of chromosomal loci responsible for mucin metabolism that appear to be under common transcriptional control and with predicted functions that allow degradation of various O-linked glycans in mucin. Conservation of the latter gene clusters in various B. bifidum strains supports the notion that host-derived glycan catabolism is an important colonization factor for B. bifidum with concomitant impact on intestinal microbiota ecology.
人类肠道中密集分布着微生物群落，其代谢活动受到双歧杆菌等多种因素的影响。然而，双歧杆菌适应人类肠道的遗传基础还知之甚少。对从婴儿粪便中分离出来的双歧杆菌 Bifidobacterium bifidum PRL2010 的 2214650 碱基对基因组进行分析，揭示了一种针对宿主来源糖的营养获取策略，如粘蛋白中存在的糖。蛋白质组和转录组分析揭示了一组负责粘蛋白代谢的染色体基因座，这些基因座似乎受到共同的转录调控，具有降解粘蛋白中各种 O 连接聚糖的预测功能。各种双歧杆菌菌株中这些基因簇的保守性支持了这样一种观点，即宿主来源的聚糖分解代谢是双歧杆菌的一个重要定植因子，同时对肠道微生物组生态也有影响。
