Farkas V, Labudová I, Bauer S, Ferenczy L
Folia Microbiol (Praha). 1981;26(2):129-32. doi: 10.1007/BF02927368.
Four mutant strains exhibiting increased production of cullulases were prepared by UV irradiation of conidia of Trichoderma viride QM 9414. Selected mutants were tested for production of cellulases in submerged cultivations in shake flasks and in a 30-L fermentor in a synthetic medium containing 1 % microcrystaline cellulose as the carbon source. Some mutants showed considerable morphological differences when compared to the parent strain, the most noticeable being a higher degree of branching of the mutant hyphae. The branched mutants produced 2 to 3 times higher levels of beta-glucosidase than the parent strain QM 9414.
通过对绿色木霉QM 9414的分生孢子进行紫外线照射，制备了四种纤维素酶产量增加的突变菌株。对筛选出的突变体在摇瓶中的深层培养以及在含有1%微晶纤维素作为碳源的合成培养基中的30升发酵罐中进行纤维素酶生产测试。与亲本菌株相比，一些突变体表现出相当大的形态差异，最明显的是突变体菌丝的分支程度更高。分支突变体产生的β-葡萄糖苷酶水平比亲本菌株QM 9414高2至3倍。
