Gearing A J, Bird C R, Redhead K, Thomas M
National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, U.K.
FEMS Microbiol Immunol. 1989 Mar;1(4):205-11. doi: 10.1111/j.1574-6968.1989.tb02384.x.
We have compared the responses of peripheral blood leucocytes from three groups (i) patients suffering from pertussis (whooping cough), (ii) clinical staff caring for those patients and laboratory staff working with Bordetella pertussis, and (iii) staff with no known recent contact with B. pertussis. In vitro stimulation with filamentous haemagglutinin (FHA) caused significant increases in proliferation of only the patient group's lymphocytes. In vitro stimulation with pertussis toxin (PT) caused a large increase in proliferation of lymphocytes from all three groups and in the patient group the increase in proliferation was related to the dose of PT. Interleukin 2 (IL-2) production by leucocytes from all three groups was significantly increased following challenge with FHA or PT. The increases in IL-2 production were greatest in lymphocytes from patients with pertussis. Challenge with toxoided pertussis toxin had no effect on either proliferation or IL-2 production in any of the groups.
（i）患有百日咳（小儿咳嗽）的患者；（ii）护理这些患者的临床工作人员以及从事百日咳博德特氏菌研究的实验室工作人员；（iii）近期无已知百日咳博德特氏菌接触史的工作人员。用丝状血凝素（FHA）进行体外刺激仅使患者组的淋巴细胞增殖显著增加。用百日咳毒素（PT）进行体外刺激使所有三组的淋巴细胞增殖大幅增加，并且在患者组中，增殖的增加与PT的剂量相关。用FHA或PT刺激后，所有三组白细胞产生的白细胞介素2（IL-2）均显著增加。百日咳患者淋巴细胞中IL-2的产生增加最为显著。用类毒素化百日咳毒素刺激对任何一组的增殖或IL-2产生均无影响。
