Alías-Villegas Cynthia, Cubo M Teresa, Lara-Dampier Victoria, Bellogín Ramón A, Camacho María, Temprano Francisco, Espuny M Rosario
Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Avda. Reina Mercedes 6, Sevilla 41012, Spain.
Centro Las Torres-Tomejil (IFAPA), Apartado Oficial, Alcalá del Río, Sevilla, Spain.
Syst Appl Microbiol. 2015 Oct;38(7):506-14. doi: 10.1016/j.syapm.2015.07.003. Epub 2015 Jul 26.
The isolation and characterisation of nitrogen-fixing root nodule bacteria from Medicago marina, a tolerant legume species, were studied in two areas from southwest Spain. A total of 30 out of 82 isolates with distinct ERIC-PCR fingerprints were analysed on the basis of molecular (PCR-RFLP of the 16S-23S rDNA intergenic spacer region (IGS) with two endonucleases, analysis of the 16S rDNA and symbiotic nodC gene sequences, plasmid profiles and SDS-PAGE of LPS, including the partial sequence of the housekeeping gene glnII and the symbiotic gene nodA of some representatives), physiological (utilisation of sole carbon sources, tolerance to antibiotics, NaCl, heavy metals, temperature and pH) and symbiotic parameters (efficacy on M. marina, M. minima, M. murex, M. orbicularis, M. polymorpha, M. sativa and M. truncatula). All the bacteria isolated from M. marina nodules belonged to Ensifer meliloti, except for one strain that belonged to E. medicae. To determine the nodulation range of M. marina, 10 different Ensifer species were tested for their ability to nodulate on this plant. E. kummerowiae CCBAU 71714 and the E. medicae control strain M19.1 were the only Ensifer species tested that developed nitrogen-fixing nodules on this plant. Most of the M. marina-nodulating strains showed tolerance to stress factors and all of them shared the presence of a gene similar to cadA, a gene that encodes for a PIB-type ATPase, which is a transporter belonging to the large superfamily of ATP-driven pumps involved in the transport of metals across cell membranes.
在西班牙西南部的两个地区，对来自耐盐豆科植物滨海苜蓿（Medicago marina）的固氮根瘤菌进行了分离和特性研究。基于分子水平（用两种核酸内切酶对16S - 23S rDNA基因间隔区（IGS）进行PCR - RFLP分析、16S rDNA和共生nodC基因序列分析、质粒图谱分析以及脂多糖的SDS - PAGE分析，包括一些代表菌株的看家基因glnII和共生基因nodA的部分序列）、生理水平（唯一碳源利用情况、对抗生素、NaCl、重金属、温度和pH的耐受性）和共生参数（对滨海苜蓿、小苜蓿（M. minima）、多刺苜蓿（M. murex）、圆叶苜蓿（M. orbicularis）、多形苜蓿（M. polymorpha）、紫花苜蓿（M. sativa）和蒺藜苜蓿（M. truncatula）的有效性），对82株具有不同ERIC - PCR指纹图谱的分离株中的30株进行了分析。从滨海苜蓿根瘤中分离出的所有细菌，除了一株属于中慢生根瘤菌（E. medicae）外，均属于苜蓿中华根瘤菌（Ensifer meliloti）。为了确定滨海苜蓿的结瘤范围，测试了10种不同的中华根瘤菌属物种在该植物上结瘤的能力。鸡眼草中华根瘤菌（E. kummerowiae）CCBAU 71714和中慢生根瘤菌对照菌株M19.1是所测试的仅有的能在该植物上形成固氮根瘤的中华根瘤菌属物种。大多数能与滨海苜蓿结瘤的菌株表现出对胁迫因子的耐受性，并且它们都具有一个与cadA相似的基因，cadA基因编码一种PIB型ATP酶，该酶是一种转运蛋白，属于参与金属跨细胞膜转运的由ATP驱动的泵的大型超家族。
