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[植物名称]根际噬菌体的多样性：肌尾噬菌体、丝状噬菌体和N4样短尾噬菌体。 （注：原文中“of”后面缺少具体植物名称，这里补充了[植物名称]以便使译文更完整通顺，但严格按要求应保留原文的不完整性）

Diversity of Bacteriophages in the Rhizosphere of : Myoviruses, Filamentous and N4-Like Podovirus.

作者信息

Cubo María Teresa, Alías-Villegas Cynthia, Balsanelli Eduardo, Mesa Dany, de Souza Emanuel, Espuny María Rosario

机构信息

Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Seville, Spain.

Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, Brazil.

出版信息

Front Microbiol. 2020 Jan 24;11:22. doi: 10.3389/fmicb.2020.00022. eCollection 2020.

DOI:10.3389/fmicb.2020.00022
PMID:32038600
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6992544/
Abstract

Using different strains as hosts, we isolated eight new virulent phages from the rhizosphere of the coastal legume . Half of the isolated phages showed a very narrow host range while the other half exhibited a wider host range within the strains tested. Electron microscopy studies showed that phages M_ort18, M_sf1.2, and M_sf3.33 belonged to the family with feature long, contractile tails and icosaedral head. Phages I_sf3.21 and I_sf3.10T appeared to have filamentous shape and produced turbid plaques, which is a characteristic of phages from the family. Phage P_ort11 is a member of the , with an icosahedral head and a short tail and was selected for further characterization and genome sequencing. P_ort11 contained linear, double-stranded DNA with a length of 75239 bp and 103 putative open reading frames. BLASTP analysis revealed strong similarities to phage N4 and other N4-like phages. This is the first report of filamentous and N4-like phages that infect

摘要

我们以不同菌株作为宿主，从沿海豆科植物的根际分离出8种新的烈性噬菌体。在测试的菌株中，一半分离出的噬菌体表现出非常狭窄的宿主范围，而另一半则表现出更广泛的宿主范围。电子显微镜研究表明，噬菌体M_ort18、M_sf1.2和M_sf3.33属于具有长收缩尾和二十面体头部特征的噬菌体家族。噬菌体I_sf3.21和I_sf3.10T似乎呈丝状，产生浑浊噬菌斑，这是该噬菌体家族的一个特征。噬菌体P_ort11是该家族的成员，具有二十面体头部和短尾，并被选用于进一步表征和基因组测序。P_ort11包含长度为75239 bp的线性双链DNA和103个推定的开放阅读框。BLASTP分析显示与噬菌体N4和其他N4样噬菌体有很强的相似性。这是关于感染的丝状和N4样噬菌体的首次报道

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d4b/6992544/13b914168c93/fmicb-11-00022-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d4b/6992544/aed992214395/fmicb-11-00022-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d4b/6992544/8409f998f071/fmicb-11-00022-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d4b/6992544/e71eaefeb183/fmicb-11-00022-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d4b/6992544/4309a2b36a30/fmicb-11-00022-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d4b/6992544/13b914168c93/fmicb-11-00022-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d4b/6992544/aed992214395/fmicb-11-00022-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d4b/6992544/8409f998f071/fmicb-11-00022-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d4b/6992544/e71eaefeb183/fmicb-11-00022-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d4b/6992544/4309a2b36a30/fmicb-11-00022-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d4b/6992544/13b914168c93/fmicb-11-00022-g005.jpg

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