Schloesser Anke, Esatbeyoglu Tuba, Piegholdt Stefanie, Dose Janina, Ikuta Naoko, Okamoto Hinako, Ishida Yoshiyuki, Terao Keiji, Matsugo Seiichi, Rimbach Gerald
Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Straße 6, 24118 Kiel, Germany.
Graduate School of Medicine, Kobe University, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
Oxid Med Cell Longev. 2015;2015:789710. doi: 10.1155/2015/789710. Epub 2015 Aug 2.
Brain aging is accompanied by a decrease in mitochondrial function. In vitro studies suggest that tocotrienols, including γ- and δ-tocotrienol (T3), may exhibit neuroprotective properties. However, little is known about the effect of dietary T3 on mitochondrial function in vivo. In this study, we monitored the effect of a dietary T3/γ-cyclodextrin complex (T3CD) on mitochondrial membrane potential and ATP levels in the brain of 21-month-old mice. Mice were fed either a control diet or a diet enriched with T3CD providing 100 mg T3 per kg diet for 6 months. Dietary T3CD significantly increased mitochondrial membrane potential and ATP levels compared to those of controls. The increase in MMP and ATP due to dietary T3CD was accompanied by an increase in the protein levels of the mitochondrial transcription factor A (TFAM). Furthermore, dietary T3CD slightly increased the mRNA levels of superoxide dismutase, γ-glutamyl cysteinyl synthetase, and heme oxygenase 1 in the brain. Overall, the present data suggest that T3CD increases TFAM, mitochondrial membrane potential, and ATP synthesis in the brains of aged mice.
大脑衰老伴随着线粒体功能的下降。体外研究表明，包括γ-和δ-生育三烯酚（T3）在内的生育三烯酚可能具有神经保护特性。然而，关于饮食中T3对体内线粒体功能的影响知之甚少。在本研究中，我们监测了饮食中T3/γ-环糊精复合物（T3CD）对21月龄小鼠大脑线粒体膜电位和ATP水平的影响。给小鼠喂食对照饮食或富含T3CD的饮食，每千克饮食提供100毫克T3，持续6个月。与对照组相比，饮食中的T3CD显著增加了线粒体膜电位和ATP水平。饮食中T3CD导致的线粒体膜电位（MMP）和ATP的增加伴随着线粒体转录因子A（TFAM）蛋白水平的增加。此外，饮食中的T3CD略微增加了大脑中超氧化物歧化酶、γ-谷氨酰半胱氨酸合成酶和血红素加氧酶1的mRNA水平。总体而言，目前的数据表明，T3CD增加了老年小鼠大脑中的TFAM、线粒体膜电位和ATP合成。
