一种采用顶空固相微萃取和气相色谱-火焰离子化检测器同时测定果汁中糠醛、2-甲基糠醛和 2-戊基呋喃的高效方法。
An efficient method for the simultaneous determination of furan, 2-methylfuran and 2-pentylfuran in fruit juices by headspace solid phase microextraction and gas chromatography-flame ionisation detector.
机构信息
Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada; School of Science, Beijing University of Chemical Technology, Beijing, China.
Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
出版信息
Food Chem. 2016 Feb 1;192:9-14. doi: 10.1016/j.foodchem.2015.06.100. Epub 2015 Jun 30.
A headspace solid phase microextraction (HS-SPME) procedure followed by gas chromatography-flame ionisation detector (GC-FID) analysis was developed and validated for the simultaneous analysis of furan, 2-methylfuran and 2-pentylfuran from juice samples. Extraction at 32 °C for 20 min with stirring at 600 rpm and NaCl concentration 15% (W/V) was the optimal HS-SPME condition for all the three compounds by using a carboxen/polydimethylsiloxane fused silica fibre (75 μm). The extracted compounds were base line separated on a SPB-1 GC column within 12 min. The relative standard deviations of all analytes were less than 6.7%. The recovery rates were between 90.2% and 110.1%. The limits of detection and limits of quantification were 0.056-0.23 ng/mL and 0.14-0.76 ng/mL, respectively. The results showed that the developed method was sensitive, precise, accurate and robust for the determination of furan, 2-methylfuran and 2-pentylfuran in complex matrices without interferences from other components.
建立并验证了一种顶空固相微萃取（HS-SPME）-气相色谱-火焰离子化检测器（GC-FID）方法，用于果汁样品中糠醛、2-甲基糠醛和 2-戊基呋喃的同时分析。通过使用羧基/聚二甲基硅氧烷-熔融石英纤维（75μm），在 32°C 下搅拌 20min，搅拌速度为 600rpm，NaCl 浓度为 15%（W/V），是所有三种化合物的最佳 HS-SPME 条件。提取的化合物在 SPB-1 GC 柱上可在 12min 内实现基线分离。所有分析物的相对标准偏差均小于 6.7%。回收率在 90.2%至 110.1%之间。检测限和定量限分别为 0.056-0.23ng/mL 和 0.14-0.76ng/mL。结果表明，该方法灵敏、精确、准确且稳健，可用于复杂基质中糠醛、2-甲基糠醛和 2-戊基呋喃的测定，不受其他成分的干扰。
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