Laboratoire B.A.E, Université De Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.
Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology (CIIT), Lahore 54000, Pakistan; Laboratoire B.A.E, Université De Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.
Food Chem. 2016 Feb 1;192:799-804. doi: 10.1016/j.foodchem.2015.07.080. Epub 2015 Jul 21.
In this work, we propose for the first time a sensitive Ochratoxin A (OTA) detection in cocoa beans using competitive aptasensor by differential pulse voltammetry (DPV). In the proposed method, biotin labeled and free OTA competed to bind with immobilized aptamer onto the surface of a screen printed carbon electrode (SPCE), and percentage binding was calculated. The detection was performed after adding avidin-ALP to perform avidin-biotin reaction; the signal was generated through a suitable substrate 1-naphthyl phosphate (1-NP), for alkaline phosphatase (ALP). The cocoa samples were extracted and purified using molecular imprinted polymer (MIP) columns specifically designed for OTA. The developed aptasensor showed a good linearity in the range 0.15-5 ng/mL with the limit of detection (LOD) 0.07 ng/mL and 3.7% relative standard deviation (RSD). The aptasensor displayed good recovery values in the range 82.1-85% with 3.87% RSD, thus, demonstrated the efficiency of proposed aptasensor for such matrices.
本工作首次提出了一种利用竞争型适配体传感器通过差分脉冲伏安法（DPV）检测可可豆中赭曲霉毒素 A（OTA）的灵敏方法。在该方法中，生物素标记的游离 OTA 与固定在丝网印刷碳电极（SPCE）表面的适配体竞争结合，并计算结合百分比。在添加亲和素-碱性磷酸酶（ALP）以进行亲和素-生物素反应后进行检测；信号通过合适的 1-萘基磷酸酯（1-NP）底物产生，用于 ALP。采用专门设计用于 OTA 的分子印迹聚合物（MIP）柱从可可样品中提取和纯化。所开发的适配体传感器在 0.15-5ng/mL 范围内表现出良好的线性，检出限（LOD）为 0.07ng/mL，相对标准偏差（RSD）为 3.7%。适配体传感器在 82.1-85%的范围内显示出良好的回收率，RSD 为 3.87%，因此证明了该适配体传感器在该基质中的效率。
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