Sensitive colorimetric detection of ochratoxin A by a dual-functional Au/FeO nanohybrid-based aptasensor.

A novel colorimetric aptasensor based on a Au/FeO nanohybrid was developed to detect ochratoxin A (OTA). The aptasensor is composed of a free OTA aptamer, a Au/FeO nanohybrid coated with biotinylated complementary DNA of the OTA aptamer (biotin-cDNA-Au/FeO), and free alkaline-phosphatase-labeled streptavidin (SA-ALP). The Au/FeO nanohybrid not only immobilizes biotin-cDNA but also magnetically separates SA-ALP from the sample solution. One part of the OTA aptamer sequence hybridizes with biotin-cDNA immobilized on Au/FeO, and the left part of the OTA aptamer sequence covers the biotin and blocks the specific interaction between biotin and SA-ALP. OTA can interrupt the interaction of OTA aptamer binding to biotin-cDNA-Au/FeO and can inhibit the shielding effect of the OTA aptamer on biotin. The amount of SA-ALP that can be captured by biotin-cDNA-Au/FeO thus increases with increasing OTA concentration. Through a simple magnetic separation, the collected SA-ALP-linked Au/FeO can produce a yellow-colored solution in the presence of -nitrophenyl phosphate (-NPP). This colorimetric aptasensor can detect OTA as low as 1.15 ng mL with high specificity.