用于光片显微镜中样品限制的宏观光学捕获
Macro-optical trapping for sample confinement in light sheet microscopy.
作者信息
Yang Zhengyi, Piksarv Peeter, Ferrier David E K, Gunn-Moore Frank J, Dholakia Kishan
机构信息
SUPA, School of Physics and Astronomy, University of St. Andrews, North Haugh, St. Andrews, KY16 9SS, UK.
SUPA, School of Physics and Astronomy, University of St. Andrews, North Haugh, St. Andrews, KY16 9SS, UK ; Institute of Physics, University of Tartu, Ravila 14c, Tartu, 50411, Estonia.
出版信息
Biomed Opt Express. 2015 Jul 8;6(8):2778-85. doi: 10.1364/BOE.6.002778. eCollection 2015 Aug 1.
Light sheet microscopy is a powerful approach to construct three-dimensional images of large specimens with minimal photo-damage and photo-bleaching. To date, the specimens are usually mounted in agents such as agarose, potentially restricting the development of live samples, and also highly mobile specimens need to be anaesthetized before imaging. To overcome these problems, here we demonstrate an integrated light sheet microscope which solely uses optical forces to trap and hold the sample using a counter-propagating laser beam geometry. Specifically, tobacco plant cells and living Spirobranchus lamarcki larvae were successfully trapped and sectional images acquired. This novel approach has the potential to significantly expand the range of applications for light sheet imaging.
光片显微镜是一种强大的方法，可用于构建大型标本的三维图像，同时将光损伤和光漂白降至最低。迄今为止，标本通常被固定在琼脂糖等介质中，这可能会限制活体样本的发展，而且高度可移动的标本在成像前需要进行麻醉。为了克服这些问题，我们在此展示了一种集成光片显微镜，该显微镜仅利用光力，通过反向传播的激光束几何结构来捕获和固定样本。具体而言，成功捕获了烟草植物细胞和活的拉氏旋鳃虫幼虫，并获取了断层图像。这种新方法有可能显著扩大光片成像的应用范围。
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