Yang Zhengyi, Prokopas Martynas, Nylk Jonathan, Coll-Lladó Clara, Gunn-Moore Frank J, Ferrier David E K, Vettenburg Tom, Dholakia Kishan
SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, KY16 9SS, UK.
SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, KY16 9SS, UK ; School of Biology, Medical and Biological Sciences Building, University of St Andrews, North Haugh, St Andrews, KY16 9TF, UK.
Biomed Opt Express. 2014 Sep 5;5(10):3434-42. doi: 10.1364/BOE.5.003434. eCollection 2014 Oct 1.
Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.
光片成像在对完整生物标本进行成像方面正迅速变得重要起来。许多最新的创新技术依赖于传播不变的贝塞尔光束或艾里光束来形成扩展光片，以便在大视场范围内提供高分辨率。将光整形以实现传播不变光束通常依赖于空间光调制器的复杂编程或专门定制的光学元件。在此，我们基于开放获取的光片显微镜OpenSPIM，对传统光片设置进行了一种直接且低成本的改进，以实现艾里光片照明。这使得宽场单光子光片成像能够为更广泛的终端用户所用。对嵌入琼脂糖中的荧光微球和斑马鱼幼体进行了成像，以展示这样一台显微镜如何能在不影响成像质量的情况下，占用空间最小且成本最低。
