Yang Na, Zhang Lixin, Sun Chaomin
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, People's Republic of China.
Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.
Antonie Van Leeuwenhoek. 2015 Nov;108(5):1107-16. doi: 10.1007/s10482-015-0564-6. Epub 2015 Aug 27.
An aerobic, Gram-stain negative, non-spore-forming and psychrotolerant bacterium, designated strain XJ109(T), was isolated from a sewage water sample collected from Xinjiang Uigur Autonomous Region, China. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain XJ109(T) represents a novel member of the family Flavobacteriaceae. The strain showed 95.5 % similarity with the 16S rRNA gene sequence of Empedobacter brevis LMG 4011(T), 95.4% with Chishuiella changwenlii BY4(T), 95.3% with Empedobacter falsenii NF 993(T) and 92.3% with Weeksella virosa DSM 16922(T). Strain XJ109(T) showed the common phenotypic and chemotaxonomic characteristics of the family Flavobacteriaceae, containing menaquinone-6 (MK-6) as the predominant respiratory quinone and iso-C17:0 3OH and iso-C15:0 as the major fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, one unidentified phospholipid and two unidentified lipids. The genomic DNA G+C content was 38.0 mol%. Strain XJ109(T) was positive for catalase and oxidase activities, and it was observed to grow at 4-30 °C (optimal 16-20 °C), pH 6.5-10.0 (optimal 7.0-7.5) and in media containing 0-2.0% (w/v) NaCl (optimal 0.5 %). On the basis of the polyphasic evidence presented, strain XJ109(T) is considered to represent a novel genus and species of the family Flavobacteriaceae, for which the name Algoriella xinjiangensis gen. nov., sp. nov. is proposed. The type strain is XJ109(T) (=CGMCC 1.10229(T)=JCM 16590(T)).
从中国新疆维吾尔自治区采集的污水水样中分离出一株需氧、革兰氏染色阴性、不形成芽孢且耐冷的细菌，命名为菌株XJ109(T)。基于16S rRNA基因序列的系统发育分析表明，菌株XJ109(T)代表黄杆菌科的一个新成员。该菌株与短稳杆菌LMG 4011(T)的16S rRNA基因序列相似性为95.5%，与长文赤水杆菌BY4(T)的相似性为95.4%，与假稳杆菌NF 993(T)的相似性为95.3%，与维罗氏韦荣菌DSM 16922(T)的相似性为92.3%。菌株XJ109(T)表现出黄杆菌科常见的表型和化学分类特征，以甲基萘醌-6（MK-6）作为主要呼吸醌，以异-C17:0 3OH和异-C15:0作为主要脂肪酸。极性脂质谱由磷脂酰乙醇胺、一种未鉴定的磷脂和两种未鉴定的脂质组成。基因组DNA G+C含量为38.0 mol%。菌株XJ109(T)的过氧化氢酶和氧化酶活性呈阳性，在4-30°C（最适温度16-20°C）、pH 6.5-10.0（最适pH 7.0-7.5）以及含0-2.0%（w/v）NaCl（最适0.5%）的培养基中生长。基于所提供的多相证据，菌株XJ109(T)被认为代表黄杆菌科的一个新属和新种，为此提出新属名新疆阿尔戈里菌属（Algoriella xinjiangensis），新种名新疆阿尔戈里菌（Algoriella xinjiangensis）。模式菌株为XJ109(T)（=CGMCC 1.10229(T)=JCM 16590(T)）。
