State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai 200237, China; School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, Shanghai 200237, China; School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
J Hazard Mater. 2015 Dec 30;300:737-744. doi: 10.1016/j.jhazmat.2015.08.014. Epub 2015 Aug 12.
Lead (Pb) and decabromodiphenyl ether (BDE209) are the mainly co-existed contaminants at e-waste recycling sites. The potential toxicity of Pb (250 μg g(-1)) to earthworm Eisenia fetida in the presence of BDE209 (1, 10 and 100 μg g(-1)) was determined during 14-d incubation period. Compared to Pb treatment alone, the co-exposure with 1 μg g(-1) BDE209 barely affected Pb uptake, subcellular partitioning and gene expression; however, histopathological changes in earthworms' body wall (epidermal, circular and longitudinal muscles) demonstrated that 10 and 100 μg g(-1) BDE209 additions enhanced Pb uptake and altered its subcellular partitioning, indicating that Pb redistributed from fractions E (cell debris) and D (metal-rich granules) to fraction C (cytosols); Additionally, BDE209 supply significantly inhibited (p<0.05) the induction of SOD (superoxide dismutase) and CAT (catalase) gene expressions (maximum down-regulation 59% for SOD gene at Pb+100 μg g(-1) BDE209 and 89% for CAT gene at Pb+10 μg g(-1) BDE209), while facilitated (p<0.05) Hsp90 (heat shock protein 90) gene expression with maximum induction rate of 120% after exposure to Pb+10 μg g(-1) BDE209. These findings illustrate the importance of considering environmental BDE209 co-exposure when assessing Pb bioaccumulation and toxicity in multi-contaminated soil ecosystems.
在电子废物回收场，铅（Pb）和十溴联苯醚（BDE209）是主要共存污染物。在 14 天的孵化期内，测定了 BDE209（1、10 和 100μg g(-1)）存在下，对蚯蚓赤子爱胜蚓的潜在毒性（250μg g(-1)）。与单独的 Pb 处理相比，1μg g(-1) BDE209 的共暴露几乎没有影响 Pb 的摄取、亚细胞分布和基因表达；然而，蚯蚓体壁（表皮、环肌和纵肌）的组织病理学变化表明，10 和 100μg g(-1) BDE209 的添加增强了 Pb 的摄取并改变了其亚细胞分布，表明 Pb 从 E（细胞碎片）和 D（富含金属的颗粒）部分重新分配到 C（胞质溶胶）部分；此外，BDE209 的供应显著抑制（p<0.05）了 SOD（超氧化物歧化酶）和 CAT（过氧化氢酶）基因表达的诱导（在 Pb+100μg g(-1) BDE209 下 SOD 基因的最大下调 59%，在 Pb+10μg g(-1) BDE209 下 CAT 基因的最大下调 89%），而促进（p<0.05）Hsp90（热休克蛋白 90）基因表达，在暴露于 Pb+10μg g(-1) BDE209 后最大诱导率为 120%。这些发现说明了在评估多污染土壤生态系统中 Pb 生物累积和毒性时，考虑环境 BDE209 共暴露的重要性。
