Suriyachadkun Chanwit, Ngaemthao Wipaporn, Chunhametha Suwanee, Sanglier Jean-Jacques
BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand.
Int J Syst Evol Microbiol. 2015 Nov;65(11):4227-4232. doi: 10.1099/ijsem.0.000562. Epub 2015 Aug 25.
A novel filamentous bacterial strain, A-T 5190T, which developed irregular sporangia at the end of sporangiophores on substrate mycelia, was isolated from dry evergreen forest soil collected in Thailand. The 16S rRNA gene sequence and phylogenetic analysis indicated that strain A-T 5190T belonged to the genus Actinoplanes and was related most closely to Actinoplanes palleronii NBRC 14916T (98.88 % similarity) and Actinoplanes rectilineatus NBRC 13941T (98.54 %). DNA-DNA relatedness values between strain A-T 5190T and its closest relatives were below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, galactose and xylose. The predominant menaquinone was MK-9(H4). The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were unsaturated fatty acid C17 : 1 and branched fatty acids iso-C16 : 0, iso-C15 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 71.9 mol%. Evidence from phenotypic, chemotaxonomic and genotypic studies indicate that strain A-T 5190T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes luteus sp. nov. is proposed. The type strain is A-T 5190T ( = BCC 41582T = NBRC 109644T).
从泰国采集的干燥常绿森林土壤中分离出一种新型丝状细菌菌株A-T 5190T，该菌株在基质菌丝体的孢囊梗末端形成不规则的孢子囊。16S rRNA基因序列和系统发育分析表明，菌株A-T 5190T属于游动放线菌属，与苍白游动放线菌NBRC 14916T（相似度98.88%）和直形游动放线菌NBRC 13941T（相似度98.54%）关系最为密切。菌株A-T 5190T与其最亲近的亲缘菌株之间的DNA-DNA相关性值低于70%。细胞壁肽聚糖含有内消旋二氨基庚二酸。全细胞糖含有鼠李糖、核糖、半乳糖和木糖。主要的甲基萘醌是MK-9(H4)。诊断性磷脂是磷脂酰乙醇胺、二磷脂酰甘油、磷脂酰甘油和磷脂酰肌醇。主要的细胞脂肪酸是不饱和脂肪酸C17 : 1和支链脂肪酸异-C16 : 0、异-C15 : 0和anteiso-C17 : 0。基因组DNA的G+C含量为71.9mol%。表型、化学分类和基因型研究的证据表明，菌株A-T 5190T代表游动放线菌属的一个新物种，为此提出新名称黄游动放线菌（Actinoplanes luteus sp. nov.）。模式菌株是A-T 5190T（=BCC 41582T=NBRC 109644T）。
