Actinobacterial Research Unit and Microbial Resource Management Unit, Scientific Instrument Center, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Department of Applied Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4033-4038. doi: 10.1099/ijs.0.052092-0. Epub 2013 May 31.
A novel actinomycete strain, designed MW4-36(T), was isolated from tropical forest soil in Nakhon Sawan Province, Thailand. Morphological and chemotaxonomic characteristics of this strain clearly demonstrated that it belongs to the genus Dactylosporangium. The strain formed finger-shaped sporangia on short sporangiophores that emerged directly from substrate hyphae. The cell-wall peptidoglycan contained glutamic acid, glycine, alanine and meso-diaminopimelic acid including 3-hydroxy-meso-diaminopimelic acid; arabinose, glucose, rhamnose, ribose and xylose were found as whole-cell sugars. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol; no phosphatidylcholine was found. The predominant menaquinones were MK-9(H8) and MK-9(H6). Mycolic acids were not detected. The predominant cellular fatty acids were iso-C15 : 0, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 72.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences also indicated that the strain should be classified in the genus Dactylosporangium and showed that the closest relative was Dactylosporangium maewongense JCM 15933(T) (99.4 % similarity). These taxonomic data revealed that strain MW4-36(T) could be readily distinguished from its phylogenetically closest relative. On the basis of these phenotypic and genotypic data, strain MW4-36(T) is considered to represent a novel species, for which the name Dactylosporangium siamense sp. nov. is proposed. The type strain is MW4-36(T) ( = BCC 34901(T) = NBRC 106093(T)).
一株新型放线菌 MW4-36(T) 是从泰国那空沙旺府的热带森林土壤中分离得到的。该菌株的形态学和化学分类特征清楚地表明它属于游动放线菌属。该菌株在直接从基质菌丝中伸出的短孢子梗上形成指状孢子囊。细胞壁肽聚糖含有谷氨酸、甘氨酸、丙氨酸和内消旋二氨基庚二酸，包括 3-羟基内消旋二氨基庚二酸；全细胞糖含有阿拉伯糖、葡萄糖、鼠李糖、核糖和木糖。诊断性磷脂为磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油和磷脂酰肌醇；未检测到磷脂酰胆碱。主要的menaquinones 为 MK-9(H8) 和 MK-9(H6)。未检测到(myco)lic acids。主要的细胞脂肪酸为 iso-C15:0、iso-C16:0、anteiso-C15:0 和 anteiso-C17:0。基因组 DNA 的 G+C 含量为 72.9 mol%。基于 16S rRNA 基因序列的系统发育分析也表明，该菌株应归类于游动放线菌属，与 Dactylosporangium maewongense JCM 15933(T)（99.4%相似度）最为接近。这些分类学数据表明，菌株 MW4-36(T) 很容易与它的系统发育最接近的亲缘关系区分开来。基于这些表型和基因型数据，菌株 MW4-36(T) 被认为代表一个新的种，建议命名为暹罗游动放线菌（Dactylosporangium siamense sp. nov.）。模式菌株为 MW4-36(T)（ = BCC 34901(T) = NBRC 106093(T)）。
