1Exercise Pathophysiology Research Laboratory, Universidade Federal do Rio Grande do Sul, Porto Alegre, BRAZIL; 2Graduate Program in Cardiology, Universidade Federal do Rio Grande do Sul, Porto Alegre, BRAZIL; 3Department of Clinical Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, BRAZIL; 4Molecular and Protein Analysis Unit, Universidade Federal do Rio Grande do Sul, Porto Alegre, BRAZIL; 5Endocrine Division of the Hospital de Clinicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, BRAZIL; and 6Internal Medicine Department, Universidade Federal do Rio Grande do Sul, Porto Alegre, BRAZIL.
Med Sci Sports Exerc. 2016 Feb;48(2):190-9. doi: 10.1249/MSS.0000000000000764.
To evaluate the acute effect of aerobic exercise (AE) and resistance exercise (RE) on the release of endothelial progenitor cell (EPCs, CD34+/KDR+/CD45 dim) and vascular function in type 1 diabetes (T1DM).
Fourteen men with T1DM and 5 nondiabetic controls were randomly assigned to 40-min AE (60% VO 2peak) and RE sessions (60% 1-RM). The study had a crossover design, and interventions were 1 wk apart. Venous occlusion plethysmography (blood flow, reactive hyperemia, and vascular resistance) and blood collection (EPC levels, flow cytometry) were done immediately before and after exercise sessions.
Patients were 30.3 ± 1.6 yr-old, HbA1c 7.7% ± 0.2%; controls were 26.8 ± 2.3 yr-old. Groups did not differ in EPC levels at baseline or in relation to exercise. Over time, exercise did not induce changes in patients with T1DM, whereas, in controls, EPCs were decreased after AE (-10.7%, P = 0.017) and increased after RE (+12.2%, P = 0.004). Compared with baseline, blood flow increased and vascular resistance decreased after RE in both groups. Reactive hyperemia was increased 10 min after AE and RE sessions in patients with T1DM (36.5% and 42.0%, respectively) and in controls (35.4% and 74.3%), but no group differences were observed between groups in response to exercise.
Despite the increased vascular reactivity in both groups after both exercise sessions, EPCs were only influenced by exercise in controls. The unchanged number of EPCs in T1DM after exercise sessions might indicate a blunted endothelium regenerating capacity, revealing an early deterioration of the functional arterial characteristics not disclosed by only evaluating vascular functional variables.
评估有氧运动（AE）和抗阻运动（RE）对 1 型糖尿病（T1DM）患者内皮祖细胞（EPCs，CD34+/KDR+/CD45dim）释放和血管功能的急性影响。
14 名 T1DM 男性和 5 名非糖尿病对照者被随机分为 40 分钟的 AE（60%VO2peak）和 RE 组（60%1-RM）。研究采用交叉设计，干预措施相隔 1 周。静脉阻塞体积描记术（血流、反应性充血和血管阻力）和采血（EPC 水平，流式细胞术）在运动前后立即进行。
患者年龄为 30.3±1.6 岁，HbA1c 为 7.7%±0.2%；对照组年龄为 26.8±2.3 岁。两组在基线或运动时的 EPC 水平均无差异。随着时间的推移，T1DM 患者的运动并未引起变化，而对照组的 AE 后 EPC 减少（-10.7%，P=0.017），RE 后增加（+12.2%，P=0.004）。与基线相比，两组患者在 RE 后血流增加，血管阻力降低。AE 和 RE 后 10 分钟，T1DM 患者的反应性充血增加（分别为 36.5%和 42.0%）和对照组（分别为 35.4%和 74.3%），但两组之间在运动反应方面没有差异。
尽管两组在两次运动后血管反应性均增加，但仅在对照组中 EPC 受到运动的影响。运动后 T1DM 患者的 EPC 数量没有变化，可能表明内皮再生能力减弱，这揭示了仅评估血管功能变量所不能揭示的功能性动脉特征的早期恶化。
