Chaudhary Omkar, Kumar Sanjeev, Bala Manju, Singh Jasbir, Hazarika Anjali, Luthra Kalpana
1 Department of Biochemistry, Cardiothoracic and Neurosciences Center, All India Institute of Medical Sciences , New Delhi, India .
2 Regional STD Teaching Training and Research Centre, Safdarjung Hospital , New Delhi, India .
Viral Immunol. 2015 Oct;28(8):472-5. doi: 10.1089/vim.2014.0148. Epub 2015 Aug 27.
Dendritic cell-specific intracellular adhesion molecule 3 grabbing nonintegrin related molecule (DC-SIGNR) is a C-type lectin, calcium-dependent carbohydrate-binding protein, which can act as a cell-adhesion and pathogen recognition receptor. DC-SIGNR is known to be highly expressed on liver sinusoidal cells and in the lymph nodes. However, its expression in peripheral blood mononuclear cells (PBMCs) in HIV-1 infection has not been addressed. Therefore, this study determined the expression of DC-SIGNR in PBMCs of HIV-1-infected patients and healthy seronegative individuals by real-time polymerase chain reaction and assessed its correlation with CD4+ T cell counts and DC-SIGNR genotypes. A significantly higher expression of DC-SIGNR was observed in the PBMCs of HIV-1-infected patients compared with healthy seronegative individuals. Further, there was a negative correlation between DC-SIGNR expression and CD4+ T cell counts and positive with viral load, with higher DC-SIGNR expression in the PBMCs of HIV-1-infected patients with a CD4+ T cell count <200 cells/μL than those with >200 cells/μL. This is the first study to report the expression of DC-SIGNR in PBMCs of HIV-1-infected patients. A salient finding of this study is that the DC-SIGNR expression was higher in HIV-1-infected patients, and its positive correlation with viral load and negative with CD4+ T cells counts suggesting a potential role of DC-SIGNR in HIV-1 infection.
树突状细胞特异性细胞内黏附分子3结合非整合素相关分子（DC-SIGNR）是一种C型凝集素，即钙依赖性碳水化合物结合蛋白，可作为细胞黏附及病原体识别受体。已知DC-SIGNR在肝窦状细胞和淋巴结中高表达。然而，其在HIV-1感染患者外周血单个核细胞（PBMC）中的表达情况尚未见报道。因此，本研究通过实时聚合酶链反应测定了HIV-1感染患者和健康血清阴性个体PBMC中DC-SIGNR的表达，并评估了其与CD4+T细胞计数及DC-SIGNR基因型的相关性。与健康血清阴性个体相比，HIV-1感染患者的PBMC中DC-SIGNR表达显著更高。此外，DC-SIGNR表达与CD4+T细胞计数呈负相关，与病毒载量呈正相关，CD4+T细胞计数<200个/μL的HIV-1感染患者PBMC中DC-SIGNR表达高于CD4+T细胞计数>200个/μL的患者。这是首次报道HIV-1感染患者PBMC中DC-SIGNR表达的研究。本研究的一个显著发现是，HIV-1感染患者中DC-SIGNR表达更高，其与病毒载量呈正相关，与CD4+T细胞计数呈负相关，提示DC-SIGNR在HIV-1感染中可能发挥作用。
