Wang Xiangdong, Jiang Yangmei, Yuan Menglang, Chen Chunlin, Wang Keyong, Zhang Qianshi, Zuo Yunfei, Ren Shuangyi
Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China.
Department of Clinical Laboratory, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
Oncol Lett. 2017 Sep;14(3):2813-2821. doi: 10.3892/ol.2017.6508. Epub 2017 Jun 30.
Dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin-related protein (DC-SIGNR) is a type II transmembrane protein that has been reported to bind to various pathogens and participate in immunoregulation and tumorigenesis. However, further research is required to investigate whether the level of DC-SIGNR and cervical cancer are associated. The present study aimed to explore the clinical diagnostic significance of DC-SIGNR in cervical cancer. Immunohistochemical staining of DC-SIGNR was performed in samples from 25 patients with early stage cervical cancer, 14 patients with cervical intraepithelial neoplasia (CIN) and cervical polyp samples from 15 individuals. DC-SIGNR expression in cervical cancer tissue was significantly higher compared with that in CIN and cervical polyp tissue (P=0.0184 and P=0.0236, respectively). However, there was no significant difference in DC-SIGNR expression between CIN and cervical polyp tissue (P=0.8103). Additionally, the serum DC-SIGNR levels in 84 cervical cancer patients and 69 healthy female individuals were measured using an ELISA. Serum (s)DC-SIGNR levels were significantly higher in cervical cancer patients compared with healthy female individuals (P<0.0001). A sDC-SIGNR level of 93.7 ng/ml was revealed by receiver operating characteristic curve analysis to predict the presence of cervical cancer with 69.57% sensitivity and 66.67% specificity (area under the curve, 0.6989; P<0.0001). Levels of sDC-SIGNR in cervical cancer patients were also correlated with serum levels of squamous cell carcinoma antigen (r=0.2583; P=0.0348). The results of the present study demonstrate that DC-SIGNR is overexpressed in cervical cancer tissue, and suggest that DC-SIGNR could serve as a biomarker for the early diagnosis of cervical cancer. Nevertheless, further studies are required to demonstrate what role DC-SIGNR serves in cervical cancer.
树突状细胞特异性细胞间黏附分子-3结合非整合素相关蛋白（DC-SIGNR）是一种II型跨膜蛋白，据报道它可与多种病原体结合，并参与免疫调节和肿瘤发生。然而，DC-SIGNR水平与宫颈癌是否相关还需要进一步研究。本研究旨在探讨DC-SIGNR在宫颈癌中的临床诊断意义。对25例早期宫颈癌患者的样本、14例宫颈上皮内瘤变（CIN）患者的样本以及15例个体的宫颈息肉样本进行了DC-SIGNR的免疫组织化学染色。与CIN和宫颈息肉组织相比，宫颈癌组织中DC-SIGNR的表达显著更高（分别为P=0.0184和P=0.0236）。然而，CIN和宫颈息肉组织之间的DC-SIGNR表达没有显著差异（P=0.8103）。此外，使用酶联免疫吸附测定法检测了84例宫颈癌患者和69例健康女性个体的血清DC-SIGNR水平。宫颈癌患者的血清（s）DC-SIGNR水平显著高于健康女性个体（P<0.0001）。通过受试者工作特征曲线分析显示，sDC-SIGNR水平为93.7 ng/ml时预测宫颈癌的存在，灵敏度为69.57%，特异性为66.67%（曲线下面积，0.6989；P<0.0001）。宫颈癌患者的sDC-SIGNR水平也与鳞状细胞癌抗原的血清水平相关（r=0.2583；P=0.0348）。本研究结果表明，DC-SIGNR在宫颈癌组织中过表达，并提示DC-SIGNR可作为宫颈癌早期诊断的生物标志物。然而，还需要进一步研究来证明DC-SIGNR在宫颈癌中发挥何种作用。
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