van Norren D, van de Kraats J
Royal Netherlands Eye Hospital, Utrecht.
Vision Res. 1989;29(12):1825-30. doi: 10.1016/0042-6989(89)90163-6.
We describe a novel use of the Scanning Laser Ophthalmoscope (SLO), viz. as an imaging retinal densitometer. In our SLO a helium-neon or an argon laser beam is moved in a raster pattern over the retina; the reflected light is descanned (confocal SLO) and collected by a photomultiplier. Images of the fundus subtending 22 by 18 deg are displayed on a TV monitor. Single frames taken with 514 nm light were stored in a computer in arrays of 256 by 256 pixels and density differences between dark adapted and bleached images were calculated. With a full bleach density differences of about 0.35 were found in the center of the fovea; at retinal eccentricities of 15-20 deg we found 0.15. After selective bleaching with 633 nm light substantial density differences were only seen in the foveal area. We conclude that the confocal SLO is a very suitable instrument for imaging fundus reflectometry.
我们描述了扫描激光检眼镜（SLO）的一种新用途，即作为一种视网膜成像密度计。在我们的SLO中，氦氖或氩激光束以光栅模式在视网膜上移动；反射光被解扫描（共焦SLO）并由光电倍增管收集。在电视监视器上显示22×18度的眼底图像。用514nm光拍摄的单帧图像以256×256像素阵列的形式存储在计算机中，并计算暗适应和漂白图像之间的密度差异。在完全漂白后，在中央凹中心发现密度差异约为0.35；在视网膜偏心度为15 - 20度时，我们发现为0.15。在用633nm光进行选择性漂白后，仅在中央凹区域观察到明显的密度差异。我们得出结论，共焦SLO是用于眼底反射测量成像的非常合适的仪器。
