Smith Hugh A, Seijo Teresa E, Vallad Gary E, Peres Natalia A, Druffel Keri L
University of Florida, Gulf Coast Research and Education Center, 14625 CR 672, Wimauma, FL 33598.
Environ Entomol. 2015 Aug;44(4):1101-7. doi: 10.1093/ee/nvv095. Epub 2015 Jun 26.
Bemisia tabaci (Gennadius) biotype B transmits Tomato yellow leaf curl virus (TYLCV), which affects tomato production globally. Prompt destruction of virus reservoirs is a key component of virus management. Identification of weed hosts of TYLCV will be useful for reducing such reservoirs. The status of weeds as alternate hosts of TYLCV in Florida remains unclear. In greenhouse studies, B. tabaci adults from a colony reared on TYLCV-infected tomato were established in cages containing one of four weeds common to horticultural fields in central and south Florida. Cages containing tomato and cotton were also infested with viruliferous whiteflies as a positive control and negative control, respectively. Whitefly adults and plant tissue were tested periodically over 10 wk for the presence of TYLCV using PCR. After 10 wk, virus-susceptible tomato plants were placed in each cage to determine if whiteflies descended from the original adults were still infective. Results indicate that Bidens alba, Emilia fosbergii, and Raphanus raphanistrum are not hosts of TYLCV, and that Amaranthus retroflexus is a host.
烟粉虱（Bemisia tabaci (Gennadius)）生物型B传播番茄黄化曲叶病毒（TYLCV），该病毒影响全球番茄生产。迅速销毁病毒宿主是病毒管理的关键组成部分。鉴定TYLCV的杂草宿主将有助于减少此类宿主。在佛罗里达州，杂草作为TYLCV替代宿主的状况仍不明确。在温室研究中，将来自在感染TYLCV的番茄上饲养的烟粉虱成虫群体放置在笼子里，笼子里装有佛罗里达州中部和南部园艺田地常见的四种杂草之一。装有番茄和棉花的笼子也分别用带毒粉虱侵染作为阳性对照和阴性对照。在10周内定期使用PCR检测粉虱成虫和植物组织中是否存在TYLCV。10周后，将对病毒敏感的番茄植株放入每个笼子中，以确定源自原始成虫的粉虱是否仍具有传染性。结果表明，三叶鬼针草、一点红和野萝卜不是TYLCV的宿主，而反枝苋是宿主。
