Department of Entomology, University of Georgia, 2360 Rainwater Road, Tifton, GA 31793-5766, USA.
J Econ Entomol. 2012 Aug;105(4):1447-56. doi: 10.1603/ec11402.
Sweetpotato whitefly, Bemisia tabaci (Gennadius), and whitefly-transmitted tomato yellow leaf curl virus (TYLCV) are major threats to tomato production in the southeastern United States. TYLCV was introduced to Florida from the Caribbean islands and has spread to other southern states of the United States. In Georgia, in recent years, the incidence of TYLCV has been steadily increasing. Studies were conducted to monitor population dynamics of whiteflies in the vegetable production belt of Georgia, to evaluate TYLCV-resistant genotypes against whiteflies and TYLCV, and to assess the potential role of resistant genotypes in TYLCV epidemiology. Monitoring studies indicated that the peak incidence of whiteflies varied seasonally from year to year. In general, whitefly populations were not uniformly distributed. Tomato genotypes exhibited minor differences in their ability to support whitefly populations. TYLCV symptoms were visually undetectable in all but one resistant genotype. The infection rates (visually) in susceptible genotypes ranged from 40 to 87%. Greenhouse inoculations with viruliferous whiteflies followed by polymerase chain reaction (PCR) indicated that up to 100% of plants of resistant genotypes were infected, although predominantly symptomless. TYLCV acquisition by whiteflies from TYLCV-infected genotypes was tested by PCR; TYLCV acquisition rates from resistant genotypes were less than from susceptible genotypes. Nevertheless, this difference did not influence TYLCV transmission rates from resistant to susceptible genotypes. Results emphasize that resistant genotypes can serve as TYLCV and whitefly reservoirs and potentially influence TYLCV epidemics.
甘薯粉虱、烟粉虱（Bemisia tabaci）和粉虱传播的番茄黄曲叶病毒（TYLCV）是美国东南部番茄生产的主要威胁。TYLCV 从加勒比岛屿传入佛罗里达州，并已传播到美国其他南部各州。在佐治亚州，近年来，TYLCV 的发病率一直在稳步上升。本研究旨在监测佐治亚州蔬菜种植带粉虱的种群动态，评估对粉虱和 TYLCV 具有抗性的基因型，以及评估抗性基因型在 TYLCV 流行病学中的潜在作用。监测研究表明，粉虱的高峰期随年份的不同而呈季节性变化。一般来说，粉虱种群的分布不均匀。番茄基因型在支持粉虱种群方面的能力存在微小差异。除了一个抗性基因型外，所有基因型的 TYLCV 症状均肉眼不可见。敏感基因型的感染率（肉眼）在 40%至 87%之间不等。用带毒粉虱进行温室接种，然后进行聚合酶链反应（PCR）表明，尽管主要是无症状的，但高达 100%的抗性基因型植株受到感染。通过 PCR 测试了粉虱从感染 TYLCV 的基因型中获得 TYLCV 的情况；从抗性基因型中获得 TYLCV 的比率低于从敏感基因型中获得的比率。然而，这种差异并没有影响 TYLCV 从抗性到敏感基因型的传播率。研究结果强调，抗性基因型可以作为 TYLCV 和粉虱的储库，并可能影响 TYLCV 的流行。
