凸起的入侵探针：用于混合序列双链DNA识别的活化双链体，具有改善的热力学和动力学特征。
Bulged Invader probes: activated duplexes for mixed-sequence dsDNA recognition with improved thermodynamic and kinetic profiles.
作者信息
Guenther Dale C, Karmakar Saswata, Hrdlicka Patrick J
机构信息
Department of Chemistry, University of Idaho, 875 Perimeter Dr, Moscow, ID 83844-2343, USA.
出版信息
Chem Commun (Camb). 2015 Oct 18;51(81):15051-4. doi: 10.1039/c5cc06264k.
Abstract
Double-stranded oligonucleotides with +1 interstrand zipper arrangements of intercalator-functionalized nucleotides are energetically activated for recognition of mixed-sequence double-stranded DNA. Incorporation of nonyl (C9) bulges at specific positions of these probes, results in more highly affine (>5-fold), faster (>4-fold) and more persistent dsDNA recognition relative to conventional Invader probes.
摘要
具有嵌入剂功能化核苷酸的 +1 链间拉链排列的双链寡核苷酸在能量上被激活，用于识别混合序列双链 DNA。在这些探针的特定位置引入壬基（C9）凸起，相对于传统的入侵探针，可实现更高亲和力（>5 倍）、更快速度（>4 倍）和更持久的双链 DNA 识别。
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