Wang Chin-Yu, Lin Bai-Ling, Chen Chung-Hsuan
Genomics Research Center, Academia Sinica, Taipei, Taiwan.
Int J Cancer. 2016 Feb 15;138(4):918-26. doi: 10.1002/ijc.29826. Epub 2015 Sep 14.
A DNA aptamer was identified against the shared tumor-specific MAGE-A3111-125 peptide antigen. The dissociation constant between the aptamer and the peptide was measured at 57 nM. Binding of the aptamer to seven types of cancer cells, melanoma, breast, colorectal, liver, lung, pancreas and oral cancer, was confirmed with flow cytometry and fluorescence imaging. Cy3-conjugated aptamers signals were specifically localized to the surface of those cancer cells. The results indicate that the DNA aptamer against the shared tumor-specific MAGE-A3 peptide can be used in cancer cell targeting and has the potential for developing into new modalities for the diagnosis of multiple cancers.
一种针对共同肿瘤特异性MAGE - A3 111 - 125肽抗原的DNA适配体被鉴定出来。该适配体与肽之间的解离常数经测定为57 nM。通过流式细胞术和荧光成像证实了该适配体与七种癌细胞（黑色素瘤、乳腺癌、结直肠癌、肝癌、肺癌、胰腺癌和口腔癌）的结合。Cy3缀合的适配体信号特异性定位于这些癌细胞的表面。结果表明，针对共同肿瘤特异性MAGE - A3肽的DNA适配体可用于癌细胞靶向，并且有潜力发展成为多种癌症诊断的新方法。
