Li Yunlong, Li Yaohui, Chen Wenping, He Fenfei, Tan Zhaobang, Zheng Jianyong, Wang Weizhong, Zhao Qingchuan, Li Jipeng
Department of Gastrointestinal Surgery, State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, China.
Oncotarget. 2015 Sep 29;6(29):27641-50. doi: 10.18632/oncotarget.4737.
Long noncoding RNAs (lncRNAs) have recently been identified to be involved in various diseases including cancer. NEAT1 is a recently identified lncRNA with its function largely unknown in human malignancy. In the present study, we investigated NEAT1 expression in 239 cases of clinical colorectal cancer specimens and matched normal tissues. Statistical methods were utilized to analyze the association of NEAT1 with clinical features, disease-free and overall survival of patients. Results showed that NEAT1 expression in colorectal cancer was up-regulated in 72.0% (172/239) cases compared with corresponding normal counterparts, and related to tumor differentiation, invasion, metastasis and TNM stage. Kaplan-Meier analysis proved that NEAT1 was associated with both disease-free survival and overall survival of patients with colorectal cancer that patients with high NEAT1 expression tend to have unfavorable outcome. Moreover, cox's proportional hazards analysis showed that high NEAT1 expression was an independent prognostic marker of poor outcome. These results provided the first evidence that the expression of NEAT1 in colorectal cancer may play an oncogenic role in colorectal cancer differentiation, invasion and metastasis. It also proved that NEAT1 may serve as an indicator of tumor recurrence and prognosis of colorectal cancer.
长链非编码RNA（lncRNAs）最近被发现与包括癌症在内的各种疾病有关。NEAT1是最近发现的一种lncRNA，其在人类恶性肿瘤中的功能 largely unknown。在本研究中，我们调查了239例临床结直肠癌标本及配对正常组织中NEAT1的表达情况。运用统计学方法分析NEAT1与患者临床特征、无病生存期和总生存期的相关性。结果显示，与相应正常组织相比，72.0%（172/239）的结直肠癌病例中NEAT1表达上调，且与肿瘤分化、侵袭、转移及TNM分期相关。Kaplan-Meier分析证明，NEAT1与结直肠癌患者的无病生存期和总生存期均相关，即NEAT1高表达的患者往往预后不良。此外，Cox比例风险分析表明，NEAT1高表达是预后不良的独立预测指标。这些结果首次证明，NEAT1在结直肠癌中的表达可能在结直肠癌的分化、侵袭和转移中发挥致癌作用。这也证明了NEAT1可能作为结直肠癌肿瘤复发和预后的一个指标。
