Wang Wanpeng, Mou Shan, Wang Ling, Zhang Minfang, Shao Xinghua, Fang Wei, Lu Renhua, Qi Chaojun, Fan Zhuping, Cao Qin, Wang Qin, Fang Yan, Ni Zhaohui
Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
Department of Health Care center, Renji Hospital, School of Medicine, Shanghai Jiaotong University.
Sci Rep. 2015 Aug 28;5:12644. doi: 10.1038/srep12644.
Systemic lupus erythematosus (SLE) is a common but severe autoimmune systemic inflammatory disease. Lupus nephritis (LN) is a serious complication of SLE,affecting up to 70% of SLE patients. Circulating microRNAs (miRNA) are emerging as biomarkers for pathological conditions and play significant roles in intercellular communication. In present research, serum samples from healthy control, early and late stage LN patients were used to analyze the expression profile of miRNAs by microarray. Subsequent study demonstrated that miR-130b-3p in serum of patients with early stage LN were significantly up-regulated when compared with healthy controls. In addition,we have also observed that the expression of a large amount of circulating microRNAs significantly decreased in patients with late stage LN. The further analysis found that the expression of serum miR-130b-3p was positively correlated with 24-hour proteinuria and renal chronicity index in patients with early stage LN.Transfection of renal tubular cellline(HK-2)with miR-130b-3p mimics can promote epithelial-mesenchymal transition (EMT). The opposite effects were observed when transfected with miR-130b-3p inhibitors. MiR-130b-3p negatively regulated ERBB2IP expression by directly targeting the 3'-UTR of ERBB2IP The circulating miR-130b-3p might serve as a biomarker and play an important role in renal damage in early stage LN patients.
系统性红斑狼疮（SLE）是一种常见但严重的自身免疫性全身性炎症性疾病。狼疮性肾炎（LN）是SLE的一种严重并发症，影响高达70%的SLE患者。循环微小RNA（miRNA）正在成为病理状况的生物标志物，并在细胞间通讯中发挥重要作用。在本研究中，使用来自健康对照、早期和晚期LN患者的血清样本，通过微阵列分析miRNA的表达谱。随后的研究表明，与健康对照相比，早期LN患者血清中的miR-130b-3p显著上调。此外，我们还观察到，晚期LN患者中大量循环微小RNA的表达显著降低。进一步分析发现，早期LN患者血清miR-130b-3p的表达与24小时蛋白尿和肾脏慢性指数呈正相关。用miR-130b-3p模拟物转染肾小管细胞系（HK-2）可促进上皮-间质转化（EMT）。用miR-130b-3p抑制剂转染时观察到相反的效果。miR-130b-3p通过直接靶向ERBB2IP的3'-UTR负调控ERBB2IP的表达。循环miR-130b-3p可能作为一种生物标志物，在早期LN患者的肾脏损伤中起重要作用。
