Chen Ping, Cui Yun, Fu Qing Yan, Lu You Yong, Fang Jing Yuan, Chen Xiao Yu
State Key Laboratory for Oncogenes and Related Genes, Key Laboratory of Gastroenterology & Hepatology, Ministry of Health, Division of Gastroenterology and Hepatology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Cancer Institute, Shanghai Institute of Digestive Disease, Shanghai, China.
Laboratory of Molecular Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital/Institute, Beijing, China.
J Dig Dis. 2015 Oct;16(10):568-74. doi: 10.1111/1751-2980.12282.
Gastric cancer (GC) is a typical type of inflammation-related tumor. The p42.3 gene is shown to be highly expressed in GC, but its association with gastritis remains unknown. We aimed to explore the relationship between gastric inflammation and p42.3 gene in vitro and in vivo.
Normal gastric epithelial cells (GES-1) were treated with Helicobacter pylori (H. pylori) and tumor necrosis factor (TNF)-α. Total cell mRNA and protein were extracted and collected, and polymerase chain reaction and Western blot were performed to determine the relative expression of p42.3 gene. In total, 291 biopsy samples from patients with chronic non-atrophic gastritis were collected and immunohistochemistry was used to measure the p42.3 protein expression. The association between p42.3 protein expression and the clinicopathological characteristics of these patients were analyzed.
Both H. pylori and TNF-α significantly enhanced the p42.3 protein expression in GES-1 cells in a time and dose-dependent manner. In addition, p42.3 gene expression was positively associated with the severity of gastric mucosal inflammation and H. pylori infection (P = 0.000). Its expression was significantly more common in severe gastric inflammation and in H. pylori-infected cases.
p42.3 gene expression is associated with gastric mucosal inflammation that can be upregulated by TNF-α and H. pylori infection.
胃癌（GC）是一种典型的炎症相关肿瘤。p42.3基因在胃癌中呈高表达，但其与胃炎的关系尚不清楚。我们旨在在体外和体内探索胃炎症与p42.3基因之间的关系。
用幽门螺杆菌（H. pylori）和肿瘤坏死因子（TNF）-α处理正常胃上皮细胞（GES-1）。提取并收集细胞总mRNA和蛋白质，进行聚合酶链反应和蛋白质免疫印迹以测定p42.3基因的相对表达。总共收集了291例慢性非萎缩性胃炎患者的活检样本，并用免疫组织化学法检测p42.3蛋白表达。分析p42.3蛋白表达与这些患者临床病理特征之间的关联。
幽门螺杆菌和TNF-α均以时间和剂量依赖性方式显著增强GES-1细胞中p42.3蛋白的表达。此外，p42.3基因表达与胃黏膜炎症的严重程度和幽门螺杆菌感染呈正相关（P = 0.000）。其表达在严重胃炎症和幽门螺杆菌感染病例中明显更常见。
p42.3基因表达与胃黏膜炎症相关，可被TNF-α和幽门螺杆菌感染上调。
