噬菌体L5整合载体以整合态和切除态之间的平衡状态存在于分枝杆菌细胞内。
Phage L5 integrating vectors are present within the Mycobacterial Cell in an equilibrium between integrated and excised states.
作者信息
Saviola Beatrice
机构信息
Basic Medical Sciences, College of Osteopathic Medicine, Western University of Health Sciences, 309 E. Second St. Pomona CA 91766.
出版信息
Cancer Ther. 2009 Jan;7:35-42.
Abstract
Integrating mycobacterial plasmids containing the phage L5 attachment site (attP) are able to insert into the mycobacterial chromosome attB site. Plasmids containing the attP site and chromosome containing the attB site are present in equilibrium between the inserted and the excised states in the presence of the phage L5 integrase.
摘要
整合有噬菌体L5附着位点（attP）的分枝杆菌质粒能够插入分枝杆菌染色体的attB位点。在噬菌体L5整合酶存在的情况下，含有attP位点的质粒和含有attB位点的染色体在插入状态和切除状态之间处于平衡。
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2
Efficient switching of mycobacteriophage L5-based integrating plasmids in Mycobacterium tuberculosis.基于分枝杆菌噬菌体L5的整合质粒在结核分枝杆菌中的高效切换
FEMS Microbiol Lett. 2003 Dec 12;229(2):211-5. doi: 10.1016/S0378-1097(03)00823-1.
3
Instability and site-specific excision of integration-proficient mycobacteriophage L5 plasmids: development of stably maintained integrative vectors.整合功能正常的分枝杆菌噬菌体L5质粒的不稳定性及位点特异性切除：稳定维持的整合载体的构建
Int J Med Microbiol. 2001 Mar;290(8):669-75. doi: 10.1016/S1438-4221(01)80004-7.
4
Assembly and activation of site-specific recombination complexes.位点特异性重组复合体的组装与激活。
Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7760-5. doi: 10.1073/pnas.140014297.
5
Identification and characterization of mycobacteriophage L5 excisionase.分枝杆菌噬菌体L5切除酶的鉴定与特性分析
Mol Microbiol. 2000 Jan;35(2):350-60. doi: 10.1046/j.1365-2958.2000.01695.x.
6
Protein-DNA complexes in mycobacteriophage L5 integrative recombination.分枝杆菌噬菌体L5整合重组中的蛋白质-DNA复合物
J Bacteriol. 1999 Jan;181(2):454-61. doi: 10.1128/JB.181.2.454-461.1999.
7
The role of supercoiling in mycobacteriophage L5 integrative recombination.超螺旋在分枝杆菌噬菌体L5整合重组中的作用。
Nucleic Acids Res. 1998 Sep 1;26(17):4012-8. doi: 10.1093/nar/26.17.4012.
8
Characterization of the mycobacteriophage L5 attachment site, attP.分枝杆菌噬菌体L5附着位点attP的特性分析
J Mol Biol. 1997 Feb 14;266(1):76-92. doi: 10.1006/jmbi.1996.0774.
9
A novel host factor for integration of mycobacteriophage L5.一种用于分枝杆菌噬菌体L5整合的新型宿主因子。
Proc Natl Acad Sci U S A. 1996 Dec 24;93(26):15411-6. doi: 10.1073/pnas.93.26.15411.
10
Positions of strand exchange in mycobacteriophage L5 integration and characterization of the attB site.分枝杆菌噬菌体L5整合过程中链交换的位置及attB位点的特征分析
J Bacteriol. 1996 Sep;178(18):5533-6. doi: 10.1128/jb.178.18.5533-5536.1996.
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