分枝杆菌噬菌体L5整合过程中链交换的位置及attB位点的特征分析
Positions of strand exchange in mycobacteriophage L5 integration and characterization of the attB site.
作者信息
Peña C E, Stoner J E, Hatfull G F
机构信息
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, USA.
出版信息
J Bacteriol. 1996 Sep;178(18):5533-6. doi: 10.1128/jb.178.18.5533-5536.1996.
Abstract
Mycobacteriophage L5 integrates into the genome of Mycobacterium smegmatis via site-specific recombination between the phage attP site and the bacterial attB site. These two sites have a 43-bp common core sequence within which strand exchange occurs and which overlaps a tRNAGly gene at attB. We show here that a 29-bp segment of DNA is necessary and sufficient for attB function and identify the positions of strand exchange.
摘要
分枝杆菌噬菌体L5通过噬菌体附着点P位点与细菌附着点B位点之间的位点特异性重组整合到耻垢分枝杆菌的基因组中。这两个位点有一个43碱基对的共同核心序列，链交换发生在该序列内，并且该序列在附着点B处与一个甘氨酰tRNA基因重叠。我们在此表明，一段29碱基对的DNA片段对于附着点B的功能是必需且足够的，并确定了链交换的位置。
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本文引用的文献
1
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Mol Microbiol. 1993 Mar;7(5):657-67. doi: 10.1111/j.1365-2958.1993.tb01157.x.
2
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J Bacteriol. 1993 Nov;175(21):6836-41. doi: 10.1128/jb.175.21.6836-6841.1993.
3
Structure of the P22 att site. Conservation and divergence in the lambda motif of recombinogenic complexes.P22附着位点的结构。重组复合物λ基序中的保守性与差异性。
J Biol Chem. 1994 Aug 12;269(32):20798-805.
4
The actinophage RP3 DNA integrates site-specifically into the putative tRNA(Arg)(AGG) gene of Streptomyces rimosus.肌动噬菌体RP3的DNA位点特异性整合到龟裂链霉菌假定的tRNA(Arg)(AGG)基因中。
Nucleic Acids Res. 1995 Jan 11;23(1):58-63. doi: 10.1093/nar/23.1.58.
5
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9
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