Levinzon Louis, Madigan Michele, Nguyen Vuong, Hasic Enisa, Conway Max, Cherepanoff Svetlana
Save Site Institute, Sydney Medical School, The University of Sydney, Sydney Eye Hospital, Sydney, New South Wales, Australia.
Ocul Oncol Pathol. 2019 Apr;5(3):153-161. doi: 10.1159/000490038. Epub 2018 Sep 18.
To determine the expression of histone deacetylase enzymes in uveal melanoma tumour cells.
This is an observational immunohistochemical study of 16 formalin-fixed, paraffin-embedded eyes enucleated for uveal melanoma between January 2001 and March 2002. Haematoxylin and eosin paraffin sections were reviewed for histopathological parameters according to the American Joint Committee on Cancer 7th edition. Sections were then immunohistochemically stained for histone deacetylases 1, 2, 3, 4 and 6 and sirtuin 2 using an automated Leica Bond II platform and Fast Red chromogen, then digitally scanned using Aperio software before assessment of staining.
Nuclear expression of histone deacetylases 1, 2, 3, 4 and 6 and of sirtuin 2 was confirmed in uveal melanoma tumour cells. In addition, the tumour cells showed cytoplasmic expression of histone deacetylases 4 and 6 and sirtuin 2. Nuclear and cytoplasmic immunostaining was also seen in intraocular tissues uninvolved by the tumour.
Uveal melanoma tumour cells express histone deacetylases 1, 2, 3, 4 and 6 and sirtuin 2, confirming potential tissue targets for histone deacetylase inhibitors.
确定组蛋白去乙酰化酶在葡萄膜黑色素瘤肿瘤细胞中的表达。
这是一项观察性免疫组织化学研究，研究对象为2001年1月至2002年3月间因葡萄膜黑色素瘤而摘除的16只经福尔马林固定、石蜡包埋的眼睛。根据美国癌症联合委员会第7版对苏木精和伊红石蜡切片进行组织病理学参数评估。然后使用自动徕卡Bond II平台和固红显色剂对切片进行组蛋白去乙酰化酶1、2、3、4和6以及沉默调节蛋白2的免疫组织化学染色，在评估染色之前使用Aperio软件进行数字扫描。
在葡萄膜黑色素瘤肿瘤细胞中证实了组蛋白去乙酰化酶1、2、3、4和6以及沉默调节蛋白2的核表达。此外，肿瘤细胞显示组蛋白去乙酰化酶4和6以及沉默调节蛋白2的细胞质表达。在未受肿瘤累及的眼内组织中也可见核和细胞质免疫染色。
葡萄膜黑色素瘤肿瘤细胞表达组蛋白去乙酰化酶1、2、3、4和6以及沉默调节蛋白2，证实了组蛋白去乙酰化酶抑制剂的潜在组织靶点。
