Sever Aviv, Abd Elkadir Amir, Matana Yosef, Gopas Jacob, Zeiri Yehuda
Department of Biomedical Engineering, Ben-Gurion University of the Negev, 84105 Beer-Sheva, Israel.
The Shraga Segal Department of Microbiology, Immunology and Genetics, Ben-Gurion University of the Negev, and the Department of Oncology, Soroka University Medical Center, 84105 Beer-Sheva, Israel.
J Biomark. 2015;2015:841245. doi: 10.1155/2015/841245. Epub 2015 Feb 23.
Melanoma is the most malignant type of skin cancer. Early detection of melanoma is thus critical for patient prognosis and survival. At present, examination by a skilled dermatologist followed by biopsy of suspicious lesions is the diagnostic gold standard. The aim of the present study was to examine an alternative and noninvasive method for the diagnosis of melanoma at an early stage. We identified and compared the volatile organic compounds (VOCs) in mouse urine and feces, before and after a subcutaneous injection of B16 melanoma cells. We identified a total of 16 VOCs in urine and 13 VOCs in feces that could serve as potential biomarkers. Statistical analysis significantly discriminated between the cancer and control groups. These results should be validated in a larger-scale animal study, after which a study could be designed in patients to develop a melanoma biomarker.
黑色素瘤是皮肤癌中最恶性的类型。因此，黑色素瘤的早期检测对于患者的预后和生存至关重要。目前，由经验丰富的皮肤科医生进行检查，随后对可疑病变进行活检是诊断的金标准。本研究的目的是研究一种用于早期诊断黑色素瘤的替代且非侵入性的方法。我们鉴定并比较了皮下注射B16黑色素瘤细胞前后小鼠尿液和粪便中的挥发性有机化合物（VOCs）。我们在尿液中总共鉴定出16种VOCs，在粪便中鉴定出13种VOCs，它们可作为潜在的生物标志物。统计分析能够显著区分癌症组和对照组。这些结果应在更大规模的动物研究中得到验证，之后可以设计针对患者的研究来开发黑色素瘤生物标志物。
