Department of Chemistry and Biochemistry, University of California , Santa Barbara, California 93106, United States.
Cancer Research Center, Sanford Burnham Prebys Medical Discovery Institute , 10901 N. Torrey Pines Road, La Jolla, California 92037, United States.
Nano Lett. 2015 Oct 14;15(10):6745-50. doi: 10.1021/acs.nanolett.5b02594. Epub 2015 Sep 1.
Surface enhanced Raman spectroscopy (SERS) is a powerful analytical technique that has been proposed as a substitute for fluorescence for biological imaging and detection but is not yet commercially utilized. The reason lies primarily in the lower intensity and poor reproducibility of most metal nanoparticle-based tags as compared to their fluorescence-based counterparts. Here, using a technique that scrupulously preserves the same number of dye molecules in both the SERS and fluorescence measurements, we show that SERS-based biotags (SBTs) with highly reproducible optical properties can be nanoengineered such that their brightness is at least equal to that of fluorescence-based tags.
表面增强拉曼光谱（SERS）是一种强大的分析技术，它已被提议作为荧光的替代品，用于生物成像和检测，但尚未在商业上得到应用。主要原因在于与荧光相比，大多数基于金属纳米粒子的标签的强度较低且重现性较差。在这里，我们使用一种严格保持 SERS 和荧光测量中相同染料分子数量的技术，表明具有高度可重现光学特性的基于 SERS 的生物标签（SBT）可以进行纳米工程设计，使得它们的亮度至少与基于荧光的标签相等。
