Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 08826, Korea.
Department of Chemistry Education, Seoul National University, Seoul 08826, Korea.
Int J Mol Sci. 2021 Feb 10;22(4):1752. doi: 10.3390/ijms22041752.
We present a template-assisted method for synthesizing nanogap shell structures for biomolecular detections based on surface-enhanced Raman scattering. The interior nanogap-containing a silver shell structure, referred to as a silver nanogap shell (Ag NGS), was fabricated on silver nanoparticles (Ag NPs)-coated silica, by adsorbing small aromatic thiol molecules on the Ag NPs. The Ag NGSs showed a high enhancement factor and good signal uniformity, using 785-nm excitation. We performed in vitro immunoassays using a prostate-specific antigen as a model cancer biomarker with a detection limit of 2 pg/mL. To demonstrate the versatility of Ag NGS nanoprobes, extracellular duplex surface-enhanced Raman scattering (SERS) imaging was also performed to evaluate the co-expression of cancer biomarkers, human epidermal growth factor-2 (HER2) and epidermal growth factor receptor (EGFR), in a non-small cell lung cancer cell line (H522). Developing highly sensitive Ag NGS nanoprobes that enable multiplex biomolecular detection and imaging can open up new possibilities for point-of-care diagnostics and provide appropriate treatment options and prognosis.
我们提出了一种基于表面增强拉曼散射的模板辅助方法，用于合成用于生物分子检测的纳米间隙壳结构。在银纳米粒子（Ag NPs）涂覆的二氧化硅上，通过吸附小分子芳香硫醇在 Ag NPs 上，制备了含有银纳米间隙壳（Ag NGS）的内部纳米间隙的银壳结构。Ag NGS 表现出高增强因子和良好的信号均匀性，使用 785nm 激发。我们使用前列腺特异性抗原作为模型癌症生物标志物进行了体外免疫测定，检测限为 2pg/mL。为了证明 Ag NGS 纳米探针的多功能性，还进行了细胞外双链表面增强拉曼散射（SERS）成像，以评估非小细胞肺癌细胞系（H522）中癌症生物标志物人表皮生长因子-2（HER2）和表皮生长因子受体（EGFR）的共表达。开发能够进行多重生物分子检测和成像的高灵敏度 Ag NGS 纳米探针，可以为即时诊断开辟新的可能性，并提供适当的治疗选择和预后。
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