应用生物素标记的DNA探针检测弯曲杆菌。

Application of a biotin-labelled DNA probe to detect Campylobacter.

作者信息

Zhou Y T, Wang S Q

机构信息

Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.

出版信息

Zentralbl Bakteriol. 1989 Dec;272(2):186-90. doi: 10.1016/s0934-8840(89)80005-2.

DOI:10.1016/s0934-8840(89)80005-2

PMID:2631735

Abstract

A procedure for direct detection of Campylobacter in feces has been developed using a biotin-labelled DNA probe. A species-specific probe (Bio-pCJ174 probe) for C. jejuni and a multivalent probe (Bio-pooled probe) for the thermotolerant Campylobacter species were developed with Bio-11-dUTP. Both probes were used to detect Campylobacter in 100 specimens of chicken feces. The coincidence rate of the results obtained by the Bio-pooled probe and cultivation was 90% and that of the Bio-pCJ 174 probe and cultivation was 96%. Identification of isolates was also carried out with both probes.

摘要

已开发出一种使用生物素标记的DNA探针直接检测粪便中弯曲杆菌的方法。用Bio-11-dUTP制备了针对空肠弯曲杆菌的种特异性探针（Bio-pCJ174探针）和针对耐热弯曲杆菌属的多价探针（Bio-混合探针）。两种探针都用于检测100份鸡粪便样本中的弯曲杆菌。Bio-混合探针检测结果与培养结果的符合率为90%，Bio-pCJ174探针与培养结果的符合率为96%。还用这两种探针进行了分离株的鉴定。