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应用生物素标记的DNA探针检测弯曲杆菌。

Application of a biotin-labelled DNA probe to detect Campylobacter.

作者信息

Zhou Y T, Wang S Q

机构信息

Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.

出版信息

Zentralbl Bakteriol. 1989 Dec;272(2):186-90. doi: 10.1016/s0934-8840(89)80005-2.

DOI:10.1016/s0934-8840(89)80005-2
PMID:2631735
Abstract

A procedure for direct detection of Campylobacter in feces has been developed using a biotin-labelled DNA probe. A species-specific probe (Bio-pCJ174 probe) for C. jejuni and a multivalent probe (Bio-pooled probe) for the thermotolerant Campylobacter species were developed with Bio-11-dUTP. Both probes were used to detect Campylobacter in 100 specimens of chicken feces. The coincidence rate of the results obtained by the Bio-pooled probe and cultivation was 90% and that of the Bio-pCJ 174 probe and cultivation was 96%. Identification of isolates was also carried out with both probes.

摘要

已开发出一种使用生物素标记的DNA探针直接检测粪便中弯曲杆菌的方法。用Bio-11-dUTP制备了针对空肠弯曲杆菌的种特异性探针（Bio-pCJ174探针）和针对耐热弯曲杆菌属的多价探针（Bio-混合探针）。两种探针都用于检测100份鸡粪便样本中的弯曲杆菌。Bio-混合探针检测结果与培养结果的符合率为90%，Bio-pCJ174探针与培养结果的符合率为96%。还用这两种探针进行了分离株的鉴定。

相似文献

1
Application of a biotin-labelled DNA probe to detect Campylobacter.应用生物素标记的DNA探针检测弯曲杆菌。
Zentralbl Bakteriol. 1989 Dec;272(2):186-90. doi: 10.1016/s0934-8840(89)80005-2.
2
Use of non-radioactive DNA probes for detection of Campylobacter jejuni and Campylobacter coli in stool specimens.使用非放射性DNA探针检测粪便标本中的空肠弯曲菌和结肠弯曲菌。
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Identification of thermotolerant Campylobacter species from poultry using an enzyme-labelled oligonucleotide DNA probe.使用酶标记寡核苷酸DNA探针从家禽中鉴定耐热弯曲杆菌菌种。
Int J Food Microbiol. 1991 Feb;12(2-3):271-4. doi: 10.1016/0168-1605(91)90078-4.
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Evaluation of culture methods and a DNA probe-based PCR assay for detection of Campylobacter species in clinical specimens of feces.用于检测粪便临床标本中弯曲杆菌属的培养方法及基于DNA探针的聚合酶链反应分析方法的评估。
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引用本文的文献

1
Identification methods for campylobacters, helicobacters, and related organisms.弯曲杆菌、螺杆菌及相关微生物的鉴定方法。
Clin Microbiol Rev. 1996 Jul;9(3):405-22. doi: 10.1128/CMR.9.3.405.