Chuma T, Yamada T, Okamoto K, Yugi H, Ohya T
Department of Veterinary Public Health, Faculty of Agriculture, Kagoshima University, Japan.
J Vet Med Sci. 1993 Dec;55(6):1027-9. doi: 10.1292/jvms.55.1027.
A direct colony hybridization method was used for the detection of Campylobacter jejuni in chicken feces. The biotin-labeled DNA prepared from the whole genome DNA of C. jejuni subsp. jejuni ATCC 33560 reacted well with homologous DNA and slightly with C. coli DNA. The method with the probe was found to be sensitive enough to detect a small number (10(2) CFU/g) of C. jejuni in chicken feces which contained a large number of background flora. It was suggested that this simple and sensitive method was useful for a wide survey of C. jejuni.
采用直接菌落杂交法检测鸡粪便中的空肠弯曲菌。从空肠弯曲菌空肠亚种ATCC 33560全基因组DNA制备的生物素标记DNA与同源DNA反应良好，与结肠弯曲菌DNA反应较弱。发现该探针方法灵敏度足以检测含有大量背景菌群的鸡粪便中少量（10²CFU/g）的空肠弯曲菌。提示这种简单而灵敏的方法可用于空肠弯曲菌的广泛检测。
