Olive D M, Johny M, Sethi S K
Department of Microbiology, Faculty of Medicine, Kuwait University.
J Clin Microbiol. 1990 Jul;28(7):1565-9. doi: 10.1128/jcm.28.7.1565-1569.1990.
A commercially available synthetic nucleic acid probe (SNAP) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting Campylobacter species. The SNAP was able to detect either 5 ng of C. jejuni DNA or 10(5) CFU of bacteria. The SNAP could also detect DNA extracted from 10(5) CFU in mock-infected stool samples. The SNAP detected C. jejuni and C. coli but showed no reactivity with C. laridis, C. fetus subsp. fetus, C. fetus subsp. venerealis, C. fennelliae, "C. upsaliensis," C. cinaedii, C. fecalis, C. hyointestinalis, C. mucosalis, or Helicobacter (Campylobacter) pylori. The SNAP also showed no cross-reactivity with other enteric pathogens. When applied to pure cultures, the SNAP detected 55 clinical isolates of C. jejuni and 11 clinical isolates of C. coli, with an accuracy of 100%. When applied directly to clinical specimens, the SNAP detected Campylobacter spp. in 19 of 23 culture-positive stool specimens (sensitivity, 82.6%; specificity, 100%). Pure cultures of the Campylobacter strains isolated from the four probe-negative, culture-positive stool specimens gave positive reactions with the SNAP. While the SNAP had excellent sensitivity and specificity for isolated bacterial colony isolates, the main limitation to the Campylobacter probe detection kit may be the sensitivity limit on direct detection of Campylobacter organisms in stools.
将一种与碱性磷酸酶偶联的市售合成核酸探针（SNAP）与检测弯曲杆菌属细菌的标准培养技术进行了比较。SNAP能够检测出5纳克空肠弯曲菌DNA或10⁵CFU细菌。SNAP还能检测从模拟感染粪便样本中10⁵CFU提取的DNA。SNAP检测出了空肠弯曲菌和大肠弯曲菌，但与拉氏弯曲菌、胎儿弯曲菌胎儿亚种、胎儿弯曲菌性病亚种、芬内利弯曲菌、“乌普萨拉弯曲菌”、犬弯曲菌、粪便弯曲菌、猪肠弯曲菌、粘膜弯曲菌或幽门螺杆菌（弯曲杆菌属）无反应。SNAP与其他肠道病原体也无交叉反应。应用于纯培养物时，SNAP检测出55株空肠弯曲菌临床分离株和11株大肠弯曲菌临床分离株，准确率为100%。直接应用于临床标本时，SNAP在23份培养阳性粪便标本中的19份中检测到弯曲杆菌属（敏感性为82.6%；特异性为100%）。从4份探针阴性、培养阳性粪便标本中分离出的弯曲杆菌菌株的纯培养物与SNAP呈阳性反应。虽然SNAP对分离的细菌菌落具有出色的敏感性和特异性，但弯曲杆菌探针检测试剂盒的主要局限性可能在于直接检测粪便中弯曲杆菌生物体的灵敏度限制。
