• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

缺乏假定ATP结合盒转运蛋白（ΔabcBA）的包囊化绵羊布鲁氏菌可保护公羊免受野生型绵羊布鲁氏菌感染。

Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams.

作者信息

Silva Ana Patrícia C, Macêdo Auricélio A, Costa Luciana F, Rocha Cláudia E, Garcia Luize N N, Farias Jade R D, Gomes Priscilla P R, Teixeira Gustavo C, Fonseca Kessler W J, Maia Andréa R F, Neves Gabriela G, Romão Everton L, Silva Teane M A, Mol Juliana P S, Oliveira Renata M, Araújo Márcio S S, Nascimento Ernane F, Martins-Filho Olindo A, Brandão Humberto M, Paixão Tatiane A, Santos Renato L

机构信息

Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.

Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.

出版信息

PLoS One. 2015 Aug 28;10(8):e0136865. doi: 10.1371/journal.pone.0136865. eCollection 2015.

DOI:10.1371/journal.pone.0136865
PMID:26317399
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4552948/
Abstract

This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams.

摘要

本研究旨在评估候选疫苗羊布鲁氏菌ΔabcBA对雄性绵羊经野生型羊布鲁氏菌实验性攻毒的保护作用。用包裹于无菌藻酸盐中的羊布鲁氏菌ΔabcBA或未包裹的疫苗菌株对雄性绵羊进行皮下免疫。在免疫后的两个月内收集血清、尿液和精液样本。然后用野生型羊布鲁氏菌（ATCC25840）对雄性绵羊进行攻毒，并将结果与未免疫且经实验性攻毒的雄性绵羊进行比较。免疫，特别是用包裹的羊布鲁氏菌ΔabcBA免疫，可预防感染、野生型羊布鲁氏菌在精液和尿液中的分泌、精液中嗜中性粒细胞的脱落以及由野生型羊布鲁氏菌参考菌株引起的临床变化、大体和微观病变。总体而言，我们的数据表明，羊布鲁氏菌ΔabcBA菌株是预防雄性绵羊由羊布鲁氏菌感染引起的布鲁氏菌病的一种非常好的疫苗菌株。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/6fad505052ac/pone.0136865.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/79968e0f31c5/pone.0136865.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/fb3c322a5821/pone.0136865.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/2872aeed8008/pone.0136865.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/3f58e30a280e/pone.0136865.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/23f3fab5a37f/pone.0136865.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/b18d203718b9/pone.0136865.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/b4844e88cb12/pone.0136865.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/6fad505052ac/pone.0136865.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/79968e0f31c5/pone.0136865.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/fb3c322a5821/pone.0136865.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/2872aeed8008/pone.0136865.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/3f58e30a280e/pone.0136865.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/23f3fab5a37f/pone.0136865.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/b18d203718b9/pone.0136865.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/b4844e88cb12/pone.0136865.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88fe/4552948/6fad505052ac/pone.0136865.g008.jpg

相似文献

1
Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams.缺乏假定ATP结合盒转运蛋白（ΔabcBA）的包囊化绵羊布鲁氏菌可保护公羊免受野生型绵羊布鲁氏菌感染。
PLoS One. 2015 Aug 28;10(8):e0136865. doi: 10.1371/journal.pone.0136865. eCollection 2015.
2
Brucella ovis lacking a species-specific putative ATP-binding cassette transporter is attenuated but immunogenic in rams.绵羊布鲁氏菌中一种具有种特异性的假定三磷酸腺苷结合盒转运蛋白的缺失株具有减毒作用，但在公羊中具有免疫原性。
Vet Microbiol. 2013 Dec 27;167(3-4):546-53. doi: 10.1016/j.vetmic.2013.09.003. Epub 2013 Sep 12.
3
Protection Provided by an Encapsulated Live Attenuated ΔabcBA Strain of Brucella ovis against Experimental Challenge in a Murine Model.绵羊种布鲁氏菌ΔabcBA基因缺失的活疫苗株对小鼠模型实验性攻毒的保护作用
Clin Vaccine Immunol. 2015 Jul;22(7):789-97. doi: 10.1128/CVI.00191-15. Epub 2015 May 6.
4
Brucella ovis mutant in ABC transporter protects against Brucella canis infection in mice and it is safe for dogs.羊布鲁氏菌 ABC 转运蛋白突变体能保护小鼠抵抗犬布鲁氏菌感染，且对犬是安全的。
PLoS One. 2020 Apr 16;15(4):e0231893. doi: 10.1371/journal.pone.0231893. eCollection 2020.
5
Brucella outer membrane complex-loaded microparticles as a vaccine against Brucella ovis in rams.负载布鲁氏菌外膜复合物的微粒作为公羊抗绵羊布鲁氏菌的疫苗。
Vaccine. 2006 Mar 10;24(11):1897-905. doi: 10.1016/j.vaccine.2005.10.042. Epub 2005 Nov 4.
6
Evaluation of particulate acellular vaccines against Brucella ovis infection in rams.评价绵羊附睾分枝杆菌感染的微粒非细胞疫苗。
Vaccine. 2010 Apr 9;28(17):3038-46. doi: 10.1016/j.vaccine.2009.10.073. Epub 2009 Nov 1.
7
Mucosal immunization with polymeric antigen BLSOmp31 using alternative delivery systems against Brucella ovis in rams.使用替代递送系统对公羊进行黏膜免疫，用聚合抗原BLSOmp31预防绵羊布鲁氏菌病。
Vet Immunol Immunopathol. 2019 Mar;209:70-77. doi: 10.1016/j.vetimm.2019.02.005. Epub 2019 Mar 2.
8
Pathogenesis of Brucella ovis in pregnant mice and protection induced by the candidate vaccine strain B. Ovis ΔabcBA.绵羊布鲁氏菌在怀孕小鼠体内的发病机制和候选疫苗株 B. Ovis ΔabcBA 诱导的保护作用。
Vaccine. 2022 Jul 30;40(32):4617-4624. doi: 10.1016/j.vaccine.2022.06.044. Epub 2022 Jun 22.
9
Rev1Δwzm vaccine candidate is safe in young and adult sheep and protects against Brucella ovis infection in rams.Rev1Δwzm 疫苗候选物在幼羊和成年羊中安全，并可预防公羊感染绵羊布鲁氏菌。
Vaccine. 2024 Sep 17;42(22):125998. doi: 10.1016/j.vaccine.2024.05.046. Epub 2024 May 27.
10
Characterization of possible correlates of protective response against Brucella ovis infection in rams immunized with the B. melitensis Rev 1 vaccine.用马尔他布鲁氏菌Rev 1疫苗免疫的公羊中，针对绵羊布鲁氏菌感染的保护性反应可能相关因素的特征分析。
Vaccine. 2009 May 18;27(23):3039-44. doi: 10.1016/j.vaccine.2009.03.046. Epub 2009 Apr 7.

引用本文的文献

1
An SNP-based diagnostic method for S2 vaccine strain infections.一种基于单核苷酸多态性的S2疫苗株感染诊断方法。
Front Vet Sci. 2025 Jun 19;12:1570220. doi: 10.3389/fvets.2025.1570220. eCollection 2025.
2
Evaluation of Brucellosis Vaccines: A Comprehensive Review.布鲁氏菌病疫苗评估：全面综述
Front Vet Sci. 2022 Jul 18;9:925773. doi: 10.3389/fvets.2022.925773. eCollection 2022.
3
Comparative Review of Brucellosis in Small Domestic Ruminants.小型家养反刍动物布鲁氏菌病的比较综述

本文引用的文献

1
Protection Provided by an Encapsulated Live Attenuated ΔabcBA Strain of Brucella ovis against Experimental Challenge in a Murine Model.绵羊种布鲁氏菌ΔabcBA基因缺失的活疫苗株对小鼠模型实验性攻毒的保护作用
Clin Vaccine Immunol. 2015 Jul;22(7):789-97. doi: 10.1128/CVI.00191-15. Epub 2015 May 6.
2
The predicted ABC transporter AbcEDCBA is required for type IV secretion system expression and lysosomal evasion by Brucella ovis.预测的ABC转运蛋白AbcEDCBA是绵羊布鲁氏菌IV型分泌系统表达和逃避溶酶体所必需的。
PLoS One. 2014 Dec 4;9(12):e114532. doi: 10.1371/journal.pone.0114532. eCollection 2014.
3
Mutants in the lipopolysaccharide of Brucella ovis are attenuated and protect against B. ovis infection in mice.
Front Vet Sci. 2022 May 12;9:887671. doi: 10.3389/fvets.2022.887671. eCollection 2022.
4
A Brucella melitensis H38ΔwbkF rough mutant protects against Brucella ovis in rams.绵羊布鲁氏菌 H38ΔwbkF 粗糙突变体能保护公羊抵抗绵羊布鲁氏菌。
Vet Res. 2022 Mar 2;53(1):16. doi: 10.1186/s13567-022-01034-z.
5
When the Going Gets Rough: The Significance of Lipopolysaccharide Phenotype in Host-Pathogen Interactions.形势艰难时：脂多糖表型在宿主-病原体相互作用中的意义
Front Microbiol. 2021 Jul 15;12:713157. doi: 10.3389/fmicb.2021.713157. eCollection 2021.
6
The Role of Neutrophils in Brucellosis.中性粒细胞在布氏杆菌病中的作用。
Microbiol Mol Biol Rev. 2020 Oct 14;84(4). doi: 10.1128/MMBR.00048-20. Print 2020 Nov 18.
7
Immunological pathways of macrophage response to infection.巨噬细胞对 感染的免疫反应途径。
Innate Immun. 2020 Oct;26(7):635-648. doi: 10.1177/1753425920958179. Epub 2020 Sep 24.
8
Disruption of pyruvate phosphate dikinase in Brucella ovis PA CO-dependent and independent strains generates attenuation in the mouse model.破环绵羊布鲁氏菌 PA 株依赖型和非依赖型丙酮酸磷酸二激酶可使该菌在小鼠模型中减毒。
Vet Res. 2020 Aug 14;51(1):101. doi: 10.1186/s13567-020-00824-7.
9
Brucella ovis mutant in ABC transporter protects against Brucella canis infection in mice and it is safe for dogs.羊布鲁氏菌 ABC 转运蛋白突变体能保护小鼠抵抗犬布鲁氏菌感染，且对犬是安全的。
PLoS One. 2020 Apr 16;15(4):e0231893. doi: 10.1371/journal.pone.0231893. eCollection 2020.
10
Rev1 wbdR tagged vaccines against Brucella ovis.Rev1 wbdR 标记疫苗抗布鲁氏菌属ovis。
Vet Res. 2019 Nov 15;50(1):95. doi: 10.1186/s13567-019-0714-3.
绵羊布鲁氏菌脂多糖突变体减毒，并能保护小鼠免受绵羊布鲁氏菌感染。
Vet Res. 2014 Jul 17;45(1):72. doi: 10.1186/s13567-014-0072-0.
4
Evaluation in mice of Brucella ovis attenuated mutants for use as live vaccines against B. ovis infection.鼠类中减毒布鲁氏菌绵羊附睾种突变株的评估，用于预防布鲁氏菌绵羊附睾种感染的活疫苗。
Vet Res. 2014 Jun 4;45(1):61. doi: 10.1186/1297-9716-45-61.
5
Clinical and pathological changes in rams experimentally infected with Actinobacillus seminis and Histophilus somni.经实验感染绵羊精液放线杆菌和睡眠嗜组织菌的公羊的临床和病理变化
ScientificWorldJournal. 2014 Jan 27;2014:241452. doi: 10.1155/2014/241452. eCollection 2014.
6
Brucella ovis lacking a species-specific putative ATP-binding cassette transporter is attenuated but immunogenic in rams.绵羊布鲁氏菌中一种具有种特异性的假定三磷酸腺苷结合盒转运蛋白的缺失株具有减毒作用，但在公羊中具有免疫原性。
Vet Microbiol. 2013 Dec 27;167(3-4):546-53. doi: 10.1016/j.vetmic.2013.09.003. Epub 2013 Sep 12.
7
Evaluation of DNA extraction protocols for Brucella abortus pcr detection in aborted fetuses or calves born from cows experimentally infected with strain 2308.评估用于从感染 2308 株牛布鲁氏菌流产胎儿或小牛中检测布鲁氏菌 abortus pcr 的 DNA 提取方案。
Braz J Microbiol. 2009 Jul;40(3):480-9. doi: 10.1590/S1517-838220090003000010. Epub 2009 Sep 1.
8
A history of the development of Brucella vaccines.布鲁氏菌疫苗的发展历史。
Biomed Res Int. 2013;2013:743509. doi: 10.1155/2013/743509. Epub 2013 Jun 3.
9
Recent developments in livestock and wildlife brucellosis vaccination.家畜和野生动物布鲁氏菌病疫苗接种的最新进展。
Rev Sci Tech. 2013 Apr;32(1):207-17. doi: 10.20506/rst.32.1.2201.
10
Pathogenesis and pathobiology of brucellosis in livestock.家畜布鲁氏菌病的发病机制与病理生物学
Rev Sci Tech. 2013 Apr;32(1):105-15. doi: 10.20506/rst.32.1.2193.