缺乏假定ATP结合盒转运蛋白（ΔabcBA）的包囊化绵羊布鲁氏菌可保护公羊免受野生型绵羊布鲁氏菌感染。
Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams.
作者信息
Silva Ana Patrícia C, Macêdo Auricélio A, Costa Luciana F, Rocha Cláudia E, Garcia Luize N N, Farias Jade R D, Gomes Priscilla P R, Teixeira Gustavo C, Fonseca Kessler W J, Maia Andréa R F, Neves Gabriela G, Romão Everton L, Silva Teane M A, Mol Juliana P S, Oliveira Renata M, Araújo Márcio S S, Nascimento Ernane F, Martins-Filho Olindo A, Brandão Humberto M, Paixão Tatiane A, Santos Renato L
机构信息
Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
出版信息
PLoS One. 2015 Aug 28;10(8):e0136865. doi: 10.1371/journal.pone.0136865. eCollection 2015.
This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams.
本研究旨在评估候选疫苗羊布鲁氏菌ΔabcBA对雄性绵羊经野生型羊布鲁氏菌实验性攻毒的保护作用。用包裹于无菌藻酸盐中的羊布鲁氏菌ΔabcBA或未包裹的疫苗菌株对雄性绵羊进行皮下免疫。在免疫后的两个月内收集血清、尿液和精液样本。然后用野生型羊布鲁氏菌（ATCC25840）对雄性绵羊进行攻毒，并将结果与未免疫且经实验性攻毒的雄性绵羊进行比较。免疫，特别是用包裹的羊布鲁氏菌ΔabcBA免疫，可预防感染、野生型羊布鲁氏菌在精液和尿液中的分泌、精液中嗜中性粒细胞的脱落以及由野生型羊布鲁氏菌参考菌株引起的临床变化、大体和微观病变。总体而言，我们的数据表明，羊布鲁氏菌ΔabcBA菌株是预防雄性绵羊由羊布鲁氏菌感染引起的布鲁氏菌病的一种非常好的疫苗菌株。
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