Liu Cuicui, Deng Qiliang, Fang Guozhen, Huang Xuan, Wang Shuo, He Jinsong
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology , Tianjin 300457, China.
Institute of Food Science and Technology, Yunnan Agricultural University , Yunnan 650201, China.
ACS Appl Mater Interfaces. 2015 Sep 16;7(36):20430-7. doi: 10.1021/acsami.5b07668. Epub 2015 Sep 3.
Currently, many types of affinity materials have been developed for the enrichment of glycoproteins potentially considered to be clinical biomarkers; however, they can not effectively distinguish between different glycoproteins and thus lack the functionality that may be the key to the diagnosis of specific diseases. In the present work, a novel interface-free 2D monolithic material has been developed for the separation of multiple types of glycoproteins, in which boronate-functionalized graphene acts as preconcentration segment and poly(guanidinium ionic liquid) acts as separation segment. The resultant 2D material was characterized by X-ray photoelectron spectroscopy, elemental analysis, and electroosmotic flow analysis to demonstrate successful modification at each step. The performance of this 2D material was evaluated by capillary electrochromatography and allowed the successful online concentration and separation of five standard glycoproteins. The high separation efficiency can be largely attributed to the good orthogonality of boronate-functionalized graphene monolith and poly(guanidinium ionic liquid) monolith.
目前，已经开发出多种亲和材料用于富集可能被视为临床生物标志物的糖蛋白；然而，它们无法有效区分不同的糖蛋白，因此缺乏可能是特定疾病诊断关键的功能。在本研究中，开发了一种新型的无界面二维整体材料用于分离多种类型的糖蛋白，其中硼酸酯功能化的石墨烯作为预浓缩段，聚（胍离子液体）作为分离段。所得二维材料通过X射线光电子能谱、元素分析和电渗流分析进行表征，以证明每个步骤的修饰成功。通过毛细管电色谱对这种二维材料的性能进行评估，实现了五种标准糖蛋白的成功在线浓缩和分离。高分离效率很大程度上归因于硼酸酯功能化石墨烯整体柱和聚（胍离子液体）整体柱的良好正交性。
