Kurebayashi Hidetaka, Goi Takanori, Shimada Michiaki, Tagai Noriyuki, Naruse Takayuki, Nakazawa Toshiyuki, Kimura Youhei, Hirono Yasuo, Yamaguchi Akio
Department of Surgery, University of Fukui, Fukui 9101193, Japan.
Oncotarget. 2015 Sep 22;6(28):26242-51. doi: 10.18632/oncotarget.4385.
The Prokineticin 2 (PROK2) is correlated with indispensable in maintaining the homeostasis of healthy human tissues. Herein, we examined the role of PROK2 in human colorectal cancer.After total RNA extraction from 6 colorectal cancer cell lines, we examined the expression of PROK2 mRNA. For investigating angiogenesis and tumor growth in mice, the PROK2 gene was transfected into colorectal cancer cell lines having low PROK2 mRNA expression. In addition, small interfering RNA (siRNA) was transfected into colorectal cancer cell lines having high PROK2 mRNA expression for investigation of angiogenesis and tumor growth in mice.From 6 colorectal cancer cell lines studied, PROK2 mRNA expression was increased in 3 cell lines. When the PROK2 gene was transfected into the colorectal cancer cell line with low PROK2 mRNA expression, angiogenesis and tumor growth in mice increased significantly compared to the cell line with the control vector.When PROK2 siRNA was transfected into colorectal cancer cell lines with high PROK2 mRNA expression, angiogenesis and tumor growth in mice were suppressed significantly compared to the cell line with siRNA (control).This is the first report of the association of PROK2 as an angiogenic growth factor in colorectal cancer.
促动力蛋白2（PROK2）与维持健康人体组织的内环境稳定不可或缺。在此，我们研究了PROK2在人类结直肠癌中的作用。从6种结直肠癌细胞系中提取总RNA后，我们检测了PROK2 mRNA的表达。为了研究小鼠体内的血管生成和肿瘤生长情况，将PROK2基因转染到PROK2 mRNA表达较低的结直肠癌细胞系中。此外，将小干扰RNA（siRNA）转染到PROK2 mRNA表达较高的结直肠癌细胞系中，以研究小鼠体内的血管生成和肿瘤生长情况。在所研究的6种结直肠癌细胞系中，3种细胞系的PROK2 mRNA表达增加。当将PROK2基因转染到PROK2 mRNA表达较低的结直肠癌细胞系中时，与转染对照载体的细胞系相比，小鼠体内的血管生成和肿瘤生长显著增加。当将PROK2 siRNA转染到PROK2 mRNA表达较高的结直肠癌细胞系中时，与转染siRNA（对照）的细胞系相比，小鼠体内的血管生成和肿瘤生长受到显著抑制。这是关于PROK2作为结直肠癌血管生成生长因子的首次报道。
