Yeo Min Kyung, Kang Sun Hyung, Jeong Ku Bean, Lee Hyun Seok, Jeon Hong Jae, Eun Hyuk Soo, Lee Eaum Seok, Moon Hee Seok, Kim Seok Hyun, Sung Jae Kyu, Lee Byung Seok, Jeong Hyun Yong
Department of Pathology, Chungnam National University School of Medicine, Daejeon, Republic of Korea.
Division of gastroenterology and Hepatology, Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon, Republic of Korea.
Clin Med Insights Oncol. 2022 May 18;16:11795549221097941. doi: 10.1177/11795549221097941. eCollection 2022.
Although signet ring cell carcinoma (SRC) is a subtype of poorly cohesive carcinoma (PC), the differences in the biological behavior between the 2 morphologically similar carcinomas have not been fully studied. Therefore, we performed transcriptome analysis to evaluate the differences of genetic expressions between SRC and PC.
The study group consisted of patients with SRC or PC pathology from patients with early gastric cancer (EGC) whose depth of invasion was localized in the mucosal layer. A total of 18 patients were enrolled. The patients were divided into 3 groups based on their histologic type and lymph node (LN) status. Group 1 consisted of patients with PC and positive LN metastasis, Group 2 consisted of patients with PC without LN metastasis, and Group 3 consisted of patients with SRC without LN metastasis. Transcriptome analysis was performed using the nCounter PanCancer Progression Panel Kit.
The expression of 77 genes in Group 1 was altered compared to that in normal tissues. The expression of 49 and 13 genes in Groups 2 and 3, respectively, was altered when compared to that in normal tissues. Groups 1 and 2 showed similar genetic expressions. However, Group 3 showed numerous differences in gene expression including Roundabout4 (Robo4) compared to the other groups, especially Group 1.
Our data suggest that gene expression patterns were different between SRC and PC and expression of ROBO4 may play an important role in the prognosis of SRC and PC type of EGC.
虽然印戒细胞癌（SRC）是低黏附性癌（PC）的一种亚型，但这两种形态相似的癌在生物学行为上的差异尚未得到充分研究。因此，我们进行了转录组分析，以评估SRC和PC之间基因表达的差异。
研究组由早期胃癌（EGC）且浸润深度局限于黏膜层的SRC或PC病理患者组成。共纳入18例患者。根据组织学类型和淋巴结（LN）状态将患者分为3组。第1组由PC且有阳性LN转移的患者组成，第2组由PC但无LN转移的患者组成，第3组由SRC且无LN转移的患者组成。使用nCounter泛癌进展检测试剂盒进行转录组分析。
与正常组织相比，第1组中77个基因的表达发生了改变。与正常组织相比，第2组和第3组分别有49个和13个基因的表达发生了改变。第1组和第2组表现出相似的基因表达。然而，与其他组，尤其是第1组相比，第3组在基因表达上存在许多差异，包括Roundabout4（Robo4）。
我们的数据表明，SRC和PC之间的基因表达模式不同，ROBO4的表达可能在EGC的SRC和PC类型的预后中起重要作用。
