Laprise Claudie, Madathil Sreenath A, Allison Paul, Abraham Priya, Raghavendran Anantharam, Shahul Hameed P, ThekkePurakkal Akhil-Soman, Castonguay Geneviève, Coutlée François, Schlecht Nicolas F, Rousseau Marie-Claude, Franco Eduardo L, Nicolau Belinda
Division of Cancer Epidemiology, Department of Oncology, McGill University, Montreal, QC, Canada.
Division of Oral Health and Society, Faculty of Dentistry, McGill University, Montreal, QC, Canada.
Int J Cancer. 2016 Feb 15;138(4):912-7. doi: 10.1002/ijc.29827. Epub 2015 Sep 14.
Oral cancer is a major public health issue in India with ∼ 77,000 new cases and 52,000 deaths yearly. Paan chewing, tobacco and alcohol use are strong risk factors for this cancer in India. Human papillomaviruses (HPVs) are also related to a subset of head and neck cancers (HNCs). We examined the association between oral HPV and oral cancer in a sample of Indian subjects participating in a hospital-based case-control study. We recruited incident oral cancer cases (N = 350) and controls frequency-matched by age and sex (N = 371) from two main referral hospitals in Kerala, South India. Sociodemographic and behavioral data were collected by interviews. Epithelial cells were sampled using Oral CDx® brushes from the oral cancer site and the normal mucosa. Detection and genotyping of 36 HPV genotypes were done using a polymerase chain reaction protocol. Data collection procedures were performed by qualified dentists via a detailed protocol with strict quality control, including independent HPV testing in India and Canada. HPV DNA was detected in none of the cases or controls. Associations between oral cancer and risk factors usually associated with HPV infection, such as oral sex and number of lifetime sexual partners, were examined by logistic regression and were not associated with oral cancer. Lack of a role for HPV infection in this study may reflect cultural or religious characteristics specific to this region in India that are not conducive to oral HPV transmission. A nationwide representative prevalence study is needed to investigate HPV prevalence variability among Indian regions.
口腔癌是印度一个重大的公共卫生问题，每年约有77000例新发病例和52000例死亡病例。在印度，嚼槟榔、使用烟草和酒精是引发这种癌症的主要风险因素。人乳头瘤病毒（HPV）也与一部分头颈癌（HNC）有关。我们在一项基于医院的病例对照研究中，对一组印度受试者样本进行了口腔HPV与口腔癌之间关联的研究。我们从印度南部喀拉拉邦的两家主要转诊医院招募了新发口腔癌病例（N = 350）和按年龄和性别进行频率匹配的对照（N = 371）。通过访谈收集社会人口统计学和行为数据。使用Oral CDx® 刷从口腔癌部位和正常黏膜采集上皮细胞样本。采用聚合酶链反应方案对36种HPV基因型进行检测和基因分型。数据收集程序由合格的牙医按照详细方案进行，严格控制质量，包括在印度和加拿大进行独立的HPV检测。在病例组和对照组中均未检测到HPV DNA。通过逻辑回归分析了口腔癌与通常与HPV感染相关的风险因素之间的关联，如口交和终身性伴侣数量，结果发现它们与口腔癌无关。本研究中HPV感染未发挥作用，可能反映了印度该地区特有的文化或宗教特征，这些特征不利于口腔HPV的传播。需要开展一项全国代表性的患病率研究，以调查印度各地区HPV患病率的差异。
