Shafiei Seyedeh Sara, Solati-Hashjin Mehran, Samadikuchaksaraei Ali, Kalantarinejad Reza, Asadi-Eydivand Mitra, Abu Osman Noor Azuan
Department of Stem cell and Regenerative medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran 14965/161, Iran; Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia.
Biomaterials Center of Excellence, Amirkabir University of Technology, Tehran 15914, Iran; Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia.
PLoS One. 2015 Aug 28;10(8):e0136530. doi: 10.1371/journal.pone.0136530. eCollection 2015.
In recent years, nanotechnology in merging with biotechnology has been employed in the area of cancer management to overcome the challenges of chemopreventive strategies in order to gain promising results. Since most biological processes occur in nano scale, nanoparticles can act as carriers of certain drugs or agents to deliver it to specific cells or targets. In this study, we intercalated Epigallocatechin-3-Gallate (EGCG), the most abundant polyphenol in green tea, into Ca/Al-NO3 Layered double hydroxide (LDH) nanoparticles, and evaluated its efficacy compared to EGCG alone on PC3 cell line. The EGCG loaded LDH nanohybrids were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy (TEM) and nanosizer analyses. The anticancer activity of the EGCG-loaded LDH was investigated in prostate cancer cell line (PC3) while the release behavior of EGCG from LDH was observed at pH 7.45 and 4.25. Besides enhancing of apoptotic activity of EGCG, the results showed that intercalation of EGCG into LDH can improve the anti- tumor activity of EGCG over 5-fold dose advantages in in-vitro system. Subsequently, the in-vitro release data showed that EGCG-loaded LDH had longer release duration compared to physical mixture, and the mechanism of diffusion through the particle was rate-limiting step. Acidic attack was responsible for faster release of EGCG molecules from LDH at pH of 4.25 compared to pH of 7.4. The results showed that Ca/Al-LDH nanoparticles could be considered as an effective inorganic host matrix for the delivery of EGCG to PC3 cells with controlled release properties.
近年来，纳米技术与生物技术相结合已应用于癌症治疗领域，以克服化学预防策略面临的挑战，从而取得了有前景的成果。由于大多数生物过程发生在纳米尺度，纳米颗粒可作为某些药物或试剂的载体，将其递送至特定细胞或靶点。在本研究中，我们将绿茶中含量最丰富的多酚表没食子儿茶素-3-没食子酸酯（EGCG）插层到Ca/Al-NO3层状双氢氧化物（LDH）纳米颗粒中，并评估了其与单独的EGCG相比对PC3细胞系的疗效。通过X射线衍射、傅里叶变换红外光谱、透射电子显微镜（TEM）和纳米粒度分析对负载EGCG的LDH纳米杂化物进行了表征。在前列腺癌细胞系（PC3）中研究了负载EGCG的LDH的抗癌活性，同时在pH 7.45和4.25条件下观察了EGCG从LDH中的释放行为。结果表明，除了增强EGCG的凋亡活性外，将EGCG插层到LDH中可在体外系统中将EGCG的抗肿瘤活性提高5倍以上的剂量优势。随后，体外释放数据表明，与物理混合物相比，负载EGCG的LDH具有更长的释放持续时间，通过颗粒的扩散机制是限速步骤。与pH 为7.4相比，酸性攻击导致EGCG分子在pH 4.25时从LDH中更快释放。结果表明，Ca/Al-LDH纳米颗粒可被视为一种有效的无机宿主基质，用于将EGCG递送至PC3细胞并具有控释特性。
