Samidurai A J, Ware R S, Davies P S W
Children's Nutrition Research Centre, Child Health Research Centre, The University of Queensland, Herston, QLD, Australia.
School of Medicine, The University of Queensland, Herston, QLD, Australia.
Acta Paediatr. 2015 Nov;104(11):e524-9. doi: 10.1111/apa.13168.
To evaluate the effect of collecting multiple (four) urine samples on the extensive variance often observed within a cohort when determining iodine status via urinary iodine concentration (UIC).
Fifty-one children aged two to three years and thirty children aged 8-10 years participated in the study in South East Queensland, Australia. Each child's four urine samples were analysed using ammonium persulphate digestion before a Sandell-Kolthoff reaction method. Analysis of variance techniques were used to assess the effect of using multiple urine samples.
The median UICs were 223.3 and 141 μg/L for two- to three-year-olds and eight- to 10-year-olds, respectively. The coefficient of variance (CV) of UIC for children aged two to three years was reduced by 35.6%, 36.5% and 39.7% when two, three and four samples were included in the adjustment, respectively. Similarly, the CV of UIC for children aged 8-10 years was reduced by 24.7%, 30.7% and 34.7%, respectively.
Although the practicality and cost of collecting multiple UICs need to be considered, collecting multiple UIC samples from each participant provides a more accurate reflection of a cohort iodine status.
通过尿碘浓度（UIC）测定碘状态时，评估收集多个（四个）尿液样本对队列中经常观察到的广泛差异的影响。
51名2至3岁儿童和30名8至10岁儿童参与了澳大利亚昆士兰东南部的这项研究。在采用桑德尔 - 科尔托夫反应法之前，使用过硫酸铵消化法对每个儿童的四个尿液样本进行分析。采用方差分析技术评估使用多个尿液样本的影响。
2至3岁儿童和8至10岁儿童的UIC中位数分别为223.3和141μg/L。在调整中纳入两个、三个和四个样本时，2至3岁儿童UIC的变异系数（CV）分别降低了35.6%、36.5%和39.7%。同样，8至10岁儿童UIC的CV分别降低了24.7%、30.7%和34.7%。
虽然需要考虑收集多个UIC样本的实用性和成本，但从每个参与者收集多个UIC样本能更准确地反映队列的碘状态。
