Department of Laboratory Medicine, Medical University of Vienna and the General Hospital of the City of Vienna, Vienna, Austria.
Clin Chem Lab Med. 2011 Oct;49(10):1749-51. doi: 10.1515/CCLM.2011.653. Epub 2011 Jun 24.
Urinary iodide concentration (UIC) is useful to evaluate nutritional iodine status. In clinical settings UIC helps to exclude blocking of the thyroid gland by excessive endogenous iodine, if diagnostic or therapeutic administration of radio-iodine is indicated. Therefore, this study established a simple test for the measurement of UIC.
UIC was analyzed in urine samples of 200 patients. Samples were pre-treated at 95°C for 45 min with ammonium persulfate in a thermal cycler, followed by a photometric Sandell-Kolthoff reaction (SK) carried out in microtiter plates. For method comparison, UIC was analyzed in 30 samples by inductivity coupled plasma mass spectro-metry (ICP-MS) as a reference method.
Incubation conditions were optimized concerning recovery. The photometric test correlated well to the reference method (SK=0.91*ICP-MS+1, r=0.962) and presented with a functional sensitivity of 20 μg/L. UIC of patient samples ranged from <20 to 750 μg/L (median 110 μg/L); 90% of the urine samples had iodide concentrations below 210 μg/L.
The modified SK-test takes approximately 90 min for analyses of 20 urine samples compared with 27 h for ICP-MS. The photometric test provides satisfactory results and can be performed with the basic equipment of a clinical laboratory.
尿碘浓度（UIC）可用于评估营养碘状况。在临床环境中，如果需要进行放射性碘的诊断或治疗性给药，UIC 有助于排除甲状腺内源性碘过量的阻断。因此，本研究建立了一种简单的 UIC 测量方法。
对 200 例患者的尿液样本进行 UIC 分析。将样本在 95°C 下用过硫酸铵在热循环仪中预处理 45 分钟，然后在微孔板中进行比色桑德尔-科尔托夫反应（SK）。为了进行方法比较，用电感耦合等离子体质谱法（ICP-MS）对 30 个样本进行 UIC 分析作为参考方法。
优化了孵育条件以提高回收率。比色试验与参考方法相关性良好（SK=0.91*ICP-MS+1，r=0.962），功能灵敏度为 20μg/L。患者样本的 UIC 范围为<20 至 750μg/L（中位数为 110μg/L）；90%的尿液样本中碘化物浓度低于 210μg/L。
与 ICP-MS 相比，改良 SK 试验分析 20 个尿液样本大约需要 90 分钟，而 ICP-MS 则需要 27 小时。比色试验可提供满意的结果，且可在临床实验室的基本设备上进行。
