Goi Takanori, Nakazawa Toshiyuki, Hirono Yasuo, Yamaguchi Akio
First Department of Surgery, University of Fukui, 9101193, Japan.
Oncotarget. 2015 Oct 6;6(30):28790-9. doi: 10.18632/oncotarget.4744.
The angiogenic proteins vascular endothelial growth factor (VEGF) and prokineticin1 (PROK1) proteins are considered important in colorectal cancer, the relationship between their simultaneous expression and prognosis was investigated in the present study. VEGF and PROK1 expression in 620 primary human colorectal cancer lesions was confirmed via immunohistochemical staining with anti-VEGF and anti-PROK1 antibodies, and the correlation between the expression of these 2 proteins and recurrence/prognosis were investigated. VEGF protein was expressed in 329 (53.1%) and PROK1 protein was expressed in 223 (36.0%). PROK1 and VEGF were simultaneously expressed in 116 (18.7%) of the 620 cases. The correlation coefficient between VEGF expression and PROK1 expression was r = 0.11, and therefore correlation was not observed. Clinical pathology revealed that substantially lymphnode matastasis, hematogenous metastasis, or TMN advanced-stage IV was significantly more prevalent in cases that expressed both VEGF and PROK1 than in the cases negative for both proteins or those positive for only 1 of the proteins. Also the cases positive for both proteins exhibited the worst recurrence and prognosis. In the Cox proportional hazards model, VEGF and PROK1 expression was an independent prognostic factor. The prognosis was poorer in colorectal cancers that expressed both PROK1 and VEGF relative to the cases that expressed only 1 protein, and the expression of both proteins was found to be an independent prognostic factor.
血管生成蛋白血管内皮生长因子（VEGF）和促动力蛋白1（PROK1）被认为在结直肠癌中具有重要作用，本研究对它们的同时表达与预后之间的关系进行了调查。通过使用抗VEGF和抗PROK1抗体进行免疫组织化学染色，确认了620例原发性人类结直肠癌病变中VEGF和PROK1的表达，并研究了这两种蛋白表达与复发/预后之间的相关性。VEGF蛋白在329例（53.1%）中表达，PROK1蛋白在223例（36.0%）中表达。在620例病例中，116例（18.7%）同时表达PROK1和VEGF。VEGF表达与PROK1表达之间的相关系数为r = 0.11，因此未观察到相关性。临床病理显示，与两种蛋白均为阴性或仅一种蛋白为阳性的病例相比，同时表达VEGF和PROK1的病例中，实质性淋巴结转移、血行转移或TMN晚期IV期更为普遍。而且两种蛋白均为阳性的病例复发和预后最差。在Cox比例风险模型中，VEGF和PROK1表达是独立的预后因素。相对于仅表达一种蛋白的病例，同时表达PROK1和VEGF的结直肠癌预后较差，且发现两种蛋白的表达是独立的预后因素。
