（13）产油真菌卷枝毛霉中脂质积累的 C 代谢通量分析。
(13)C-metabolic flux analysis of lipid accumulation in the oleaginous fungus Mucor circinelloides.
机构信息
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; Synergistic Innovation Center for Food Safety and Nutrition, Wuxi 214122, PR China.
出版信息
Bioresour Technol. 2015 Dec;197:23-9. doi: 10.1016/j.biortech.2015.08.035. Epub 2015 Aug 20.
The oleaginous fungus Mucor circinelloides is of industrial interest because it can produce high levels of polyunsaturated fatty acid γ-linolenic acid. M. circinelloides CBS 277.49 is able to accumulate less than 15% of cell dry weight as lipids, while M. circinelloides WJ11 can accumulate lipid up to 36%. In order to better understand the mechanisms behind the differential lipid accumulation in these two strains, tracer experiments with (13)C-glucose were performed with the growth of M. circinelloides and subsequent gas chromatography-mass spectrometric detection of (13)C-patterns in proteinogenic amino acids was carried out to identify the metabolic network topology and estimate intracellular fluxes. Our results showed that the high oleaginous strain WJ11 had higher flux of pentose phosphate pathway and malic enzyme, lower flux in tricarboxylic acid cycle, higher flux in glyoxylate cycle and ATP: citrate lyase, together, it might provide more NADPH and substrate acetyl-CoA for fatty acid synthesis.
粘性真菌少根根霉因其能够产生高水平的多不饱和脂肪酸γ-亚麻酸而具有工业应用价值。CBS 277.49 号少根根霉的细胞干重中脂质的积累量不到 15%，而 WJ11 号少根根霉可以积累高达 36%的脂质。为了更好地理解这两种菌株在脂质积累方面的差异机制，我们进行了（13）C-葡萄糖示踪实验，随着少根根霉的生长，我们进行了气相色谱-质谱检测，以鉴定蛋白氨基酸中的（13）C 模式，从而确定代谢网络拓扑结构并估算细胞内通量。我们的结果表明，高油脂菌株 WJ11 具有更高的戊糖磷酸途径和苹果酸酶通量，三羧酸循环通量较低，乙醛酸循环和 ATP：柠檬酸裂解酶通量较高，这可能为脂肪酸合成提供更多的 NADPH 和底物乙酰辅酶 A。
Zotero 插件