State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China.
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, P. R China.
J Agric Food Chem. 2020 Apr 8;68(14):4245-4251. doi: 10.1021/acs.jafc.9b08155. Epub 2020 Mar 27.
is a valuable oleaginous filamentous fungus rich in γ-linolenic acid (GLA, 18:3; n-6), which is beneficial for human health. Our previous comparative proteomic analysis between high lipid-producing WJ11 and low lipid-producing CBS 277.49 indicated that glucose 6-phosphate dehydrogenase (G6PDH) and β-isopropylmalate dehydrogenase (IPMDH) were closely involved in lipid accumulation. Transcription analysis suggested that in the strain WJ11, 61 and 62, which encode G6PDH, and , which encodes IPMDH, could be the key genes regulating lipid accumulation. To further analyze the effects of these three genes (i.e., 61, 62, and ) on lipid accumulation, we respectively overexpressed these genes from WJ11 in defective CBS 277.49 strains in this study. The results showed that overexpression of 61 and 62 genes from strain WJ11 increased the fatty acid content of cell dry weight by 23-38 and 41-47%, respectively, compared with the control strain. Furthermore, overexpression of the gene from strain WJ11 increased the fatty acid content of cell dry weight by up to 67-73%. These results suggest that 61, 62, and especially genes play important roles in regulating fatty acid synthesis in .
是一种具有重要价值的油脂丝状真菌，富含γ-亚麻酸（GLA，18：3；n-6），对人体健康有益。我们之前对高产脂菌株 WJ11 和低产脂菌株 CBS 277.49 的比较蛋白质组学分析表明，葡萄糖 6-磷酸脱氢酶（G6PDH）和β-异戊烯基苹果酸脱氢酶（IPMDH）与油脂积累密切相关。转录分析表明，在菌株 WJ11 中，编码 G6PDH 的 61 和 62 以及编码 IPMDH 的 可能是调节油脂积累的关键基因。为了进一步分析这三个基因（即 61、62 和 ）对油脂积累的影响，我们分别在本研究中将这些基因从菌株 WJ11 过表达到缺陷型 CBS 277.49 菌株中。结果表明，与对照菌株相比，来自菌株 WJ11 的 61 和 62 基因的过表达分别使细胞干重的脂肪酸含量增加了 23-38%和 41-47%。此外，来自菌株 WJ11 的 基因的过表达使细胞干重的脂肪酸含量增加了高达 67-73%。这些结果表明，61、62 尤其是 基因在调节 中的脂肪酸合成中发挥重要作用。
