Tang Xin, Chen Haiqin, Gu Zhennan, Zhang Hao, Chen Yong Q, Song Yuanda, Chen Wei
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University , Wuxi 214122, P.R. China.
Colin Ratledge Center for Microbial Lipids, School of Agriculture Engineering and Food Science, Shandong University of Technology , Zibo 255100, P.R. China.
J Agric Food Chem. 2017 Jun 21;65(24):5074-5082. doi: 10.1021/acs.jafc.7b00935. Epub 2017 Jun 12.
Mucor circinelloides is one of few oleaginous fungi that produces a useful oil rich in γ-linolenic acid, but it usually only produces <25% total lipid. Nevertheless, we isolated a new strain WJ11 that can produce up to 36% lipid of cell dry weight. In this study, we have systematically analyzed the global changes in protein levels between the high lipid-producing strain WJ11 and the low lipid-producing strain CBS 277.49 (15%, lipid/cell dry weight) at lipid accumulation phase through comparative proteome analysis. Proteome analysis demonstrated that the branched-chain amino acid and lysine metabolism, glycolytic pathway, and pentose phosphate pathway in WJ11 were up-regulated, while the activities of tricarboxylic acid cycle and branch point enzyme for synthesis of isoprenoids were retarded compared with CBS 277.49. The coordinated regulation at proteome level indicate that more acetyl-CoA and NADPH are provided for fatty acid biosynthesis in WJ11 compared with CBS 277.49.
卷枝毛霉是少数能产生富含γ-亚麻酸的有用油脂的产油真菌之一，但它通常仅产生低于25%的总脂质。然而，我们分离出了一种新菌株WJ11，其脂质产量可达细胞干重的36%。在本研究中，我们通过比较蛋白质组分析，系统地分析了高产脂菌株WJ11和低产脂菌株CBS 277.49（脂质/细胞干重为15%）在脂质积累阶段蛋白质水平的全局变化。蛋白质组分析表明，与CBS 277.49相比，WJ11中的支链氨基酸和赖氨酸代谢、糖酵解途径以及磷酸戊糖途径上调，而三羧酸循环和类异戊二烯合成分支点酶的活性受到抑制。蛋白质组水平的协同调控表明，与CBS 277.49相比，WJ11为脂肪酸生物合成提供了更多的乙酰辅酶A和NADPH。
